| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hartwick, Amelia L (11)
|
NCSGSA |
43.19 |
43.15 |
-0.04 |
| 2 |
Doyle, Eva (11)
|
NCHPSC |
45.39 |
44.10 |
-1.29 |
| 3 |
Schilling, Abigail R (11)
|
NCSGSA |
44.39 |
44.59 |
0.20 |
| 4 |
Najera, Aileen F (11)
|
NCHPSC |
45.08 |
44.76 |
-0.32 |
| 5 |
O'Sullivan, Morgan S (11)
|
NCEAC |
46.59 |
45.32 |
-1.27 |
| 6 |
Proco, Chloe A (11)
|
NCEAC |
45.05 |
46.26 |
1.21 |
| 7 |
Wolff, Ameilia N (11)
|
NCSFSC |
46.75 |
47.05 |
0.30 |
| 8 |
Bateson, Halena M (11)
|
NCHPSC |
49.06 |
47.92 |
-1.14 |
| 9 |
Day, Lauren E (11)
|
NCHPSC |
48.68 |
48.46 |
-0.22 |
| 10 |
Grove, Kaye T (11)
|
NCSGSA |
49.01 |
48.84 |
-0.17 |
| 11 |
Kornatz, Maeve D (11)
|
NCEAC |
53.59 |
51.24 |
-2.35 |
| 12 |
Collins, Taylor E (11)
|
NCHPSC |
52.19 |
52.95 |
0.76 |
| 13 |
Coleman, Rori K (11)
|
NCPAST |
57.47 |
58.07 |
0.60 |
| 14 |
Hutchison, Ella A (11)
|
NCPAST |
|
1:01.21 |
- |
|
Coleman, Denise M (11)
|
NCEAC |
43.15 |
40.61 |
-2.54 |