| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cain, Amelia S (9)
|
NCPAST |
46.26 |
44.86 |
-1.40 |
| 2 |
Frank, Lillian M (9)
|
NCCAT |
48.83 |
47.40 |
-1.43 |
| 3 |
Roberts, Grace S (9)
|
NCCAT |
44.19 |
48.03 |
3.84 |
| 4 |
Najera, Aileen F (9)
|
NCHPSC |
50.24 |
48.90 |
-1.34 |
| 5 |
Schonwalder, Olivia J (9)
|
NCCAT |
53.59 |
50.11 |
-3.48 |
| 6 |
Schilling, Abigail R (9)
|
NCSGSA |
51.23 |
50.48 |
-0.75 |
| 7 |
Brinker, Abigail H (9)
|
NCNUMA |
57.98 |
54.20 |
-3.78 |
| 8 |
Silinski, Abigail A (9)
|
NCCAT |
1:08.45 |
1:03.49 |
-4.96 |
|
Salamh, Kalea L (9)
|
NCCAT |
1:10.99 |
DQ |
- |
|
Issatayeva, Nurilya K (9)
|
NCCAT |
|
NS |
- |
|
Palmer, Caroline L (9)
|
NCCAT |
55.46 |
NS |
- |
|
Romeiser, Maren A (9)
|
NCCAT |
|
NS |
- |
|
Morrow, Amelia G (9)
|
NCEAC |
37.29 |
NS |
- |
|
Britt, Olivia P (9)
|
NCNSS |
41.18 |
NS |
- |