| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Moore, Mary A (10)
|
NCTYDE |
29.21 |
29.08 |
-0.13 |
| 2 |
Zuleger, Skylar B (10)
|
NCTYDE |
31.55 |
31.77 |
0.22 |
| 3 |
Beekman, Eva L (10)
|
NCTYDE |
32.57 |
32.06 |
-0.51 |
| 4 |
Herlocker, Patterson L (10)
|
NCRACY |
36.89 |
35.35 |
-1.54 |
| 5 |
Smitherman, Katherine M (9)
|
NCTYDE |
36.34 |
36.67 |
0.33 |
| 6 |
Cvelic, Summer I (10)
|
NCTYDE |
37.64 |
38.58 |
0.94 |
| 7 |
Trevino, Gabriella B (9)
|
NCGCY |
36.88 |
38.70 |
1.82 |
| 8 |
Crider, Kendyl A (8)
|
NCTYDE |
40.03 |
40.09 |
0.06 |
| 9 |
Drozd, Charlotte A (8)
|
NCLOY |
41.88 |
40.86 |
-1.02 |
| 10 |
Lamb, Ryann M (9)
|
NCRACY |
|
44.01 |
- |
| 11 |
Whitworth, Lucy H (9)
|
NCTYDE |
42.25 |
46.99 |
4.74 |
| 12 |
Goddard, Kyla J (9)
|
NCGCY |
46.20 |
47.01 |
0.81 |
| 13 |
Wang, Ellen (9)
|
NCGCY |
49.16 |
47.05 |
-2.11 |
| 14 |
Robertson, Addilynn G (9)
|
NCTYDE |
53.17 |
52.67 |
-0.50 |
| 15 |
Gentry, Emma C (10)
|
NCLOY |
53.57 |
55.13 |
1.56 |
|
Walser, Katelynn M (10)
|
NCTYDE |
48.13 |
NS |
- |