| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Alexander, Mary Kathryn C (17)
|
NCGCY |
27.32 |
28.70 |
1.38 |
| 1 |
Frondoza, Audrianne R (12)
|
NCTYDE |
27.50 |
28.70 |
1.20 |
| 3 |
Welborn, Sophia S (11)
|
NCTYDE |
29.78 |
29.27 |
-0.51 |
| 4 |
Leatherman, Gracie N (18)
|
NCTYDE |
27.72 |
29.40 |
1.68 |
| 5 |
Davis, Bethany G (12)
|
NCTYDE |
32.25 |
30.25 |
-2.00 |
| 6 |
Copeland, Addison O (11)
|
NCTYDE |
|
30.40 |
- |
| 7 |
Whitworth, Margaret E (12)
|
NCTYDE |
32.47 |
30.64 |
-1.83 |
| 8 |
Smith, Lauren C (13)
|
NCTYDE |
32.90 |
31.42 |
-1.48 |
| 9 |
Stark, Madeleine K (11)
|
NCTYDE |
31.39 |
31.48 |
0.09 |
| 11 |
Van Noy, Jaylin L (12)
|
NCTYDE |
32.01 |
31.86 |
-0.15 |
| 13 |
Rudnicke, Hannah G (11)
|
NCTYDE |
34.35 |
34.45 |
0.10 |
| 14 |
Lang, Jenna G (12)
|
NCTYDE |
35.92 |
35.82 |
-0.10 |
| 15 |
Lewis, Piper A (12)
|
NCTYDE |
37.87 |
35.84 |
-2.03 |
| 17 |
Lack, Emilia M (12)
|
NCTYDE |
39.08 |
36.57 |
-2.51 |
| 18 |
Harris, Angelea E (10)
|
NCTYDE |
40.27 |
36.77 |
-3.50 |
| 19 |
Goodrich, Gracyn E (10)
|
NCTYDE |
37.45 |
37.89 |
0.44 |
| 21 |
Guo, Maggie Y (9)
|
NCTYDE |
41.60 |
38.68 |
-2.92 |
| 26 |
Perdue, Sophia M (8)
|
NCTYDE |
59.98 |
1:06.06 |
6.08 |