| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bollinger, Brooke A (14)
|
MALAC |
2:34.06 |
2:35.42 |
1.36 |
| 2 |
Hester, Kelly N (15)
|
MADSC |
|
2:40.93 |
- |
| 3 |
Olson, Rachel L (13)
|
MADSC |
2:39.05 |
2:41.95 |
2.90 |
| 4 |
Sudbrink, Kayla R (16)
|
MADSC |
2:47.22 |
2:46.05 |
-1.17 |
| 5 |
Harvey, Jessica (15)
|
MADSC |
2:51.40 |
2:48.18 |
-3.22 |
| 6 |
McCloskey, Grace M (14)
|
MAHAC |
2:54.01 |
2:52.85 |
-1.16 |
| 7 |
Brett, Morgan O (13)
|
MAHAC |
3:05.62 |
2:52.99 |
-12.63 |
| 8 |
Geyer, Brooke A (14)
|
MAHAC |
2:58.72 |
2:55.17 |
-3.55 |
| 9 |
Kramer, Nina E (13)
|
MALAC |
2:52.06 |
2:56.73 |
4.67 |
| 10 |
Womeldorf, Alyssa M (13)
|
MADSC |
|
2:57.81 |
- |
| 11 |
Carrasco, Katharine M (14)
|
MADSC |
3:06.13 |
2:59.02 |
-7.11 |
| 12 |
Carrasco, Carly C (14)
|
MAHAC |
3:00.15 |
3:00.68 |
0.53 |
| 13 |
Clough, Caroline E (13)
|
MAHAC |
3:05.05 |
3:01.09 |
-3.96 |
| 14 |
Yoder, Katelyn A (13)
|
MADSC |
|
3:02.40 |
- |
| 15 |
Falcon, Kiersten E (13)
|
MADSC |
|
3:15.68 |
- |
| 16 |
Kriner, Ember J (14)
|
MALAC |
3:19.58 |
3:18.11 |
-1.47 |
| 17 |
Allison, Annabelle N (14)
|
MADSC |
|
3:36.00 |
- |
|
Bieber, Brianna J (16)
|
MALAC |
2:28.98 |
NS |
- |
|
Tarkenton, Makayla F (14)
|
MAHAC |
3:13.43 |
NS |
- |
|
Campbell, Julia T (14)
|
MADSC |
3:11.82 |
NS |
- |