| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Penna, Lucy K (13)
|
SRUN |
56.77 |
56.86 |
0.09 |
| 2 |
Wong-Boulanger, Sabine Y (13)
|
IEWWSC |
59.59 |
57.76 |
-1.83 |
| 3 |
Kruse, McKinley K (13)
|
IELGSC |
1:04.80 |
1:01.75 |
-3.05 |
| 4 |
Setser, Anica M (13)
|
IELGSC |
1:04.06 |
1:02.59 |
-1.47 |
| 5 |
Raugei, Beatrice (14)
|
IETCCC |
1:04.70 |
1:02.89 |
-1.81 |
| 6 |
Munson, Sydney M (13)
|
IESAS |
1:04.21 |
1:04.28 |
0.07 |
| 7 |
LaRue, Emmeline S (13)
|
IETCCC |
1:07.79 |
1:04.34 |
-3.45 |
| 8 |
Hein, Kayla M (13)
|
IESAS |
1:04.56 |
1:06.30 |
1.74 |
| 9 |
Myers, Maddie E (13)
|
IETCCC |
1:34.83 |
1:06.87 |
-27.96 |
| 10 |
Varga, Greta V (14)
|
IETCCC |
1:07.20 |
1:07.32 |
0.12 |
| 11 |
Rutherford, Bridget H (13)
|
IETCCC |
1:08.70 |
1:08.44 |
-0.26 |
| 12 |
Goode, Makenzie J (14)
|
IESAS |
1:12.57 |
1:10.50 |
-2.07 |
| 13 |
Murray, Kennda C (13)
|
IETCCC |
|
1:12.32 |
- |
| 14 |
Ruby, Emma B (14)
|
IESAS |
1:11.96 |
1:13.08 |
1.12 |
| 15 |
Smith, Casidy M (14)
|
IESAS |
|
1:14.72 |
- |
| 16 |
Teegarden, Lily K (13)
|
IETCCC |
|
1:15.40 |
- |
| 17 |
Landon, Noelle A (14)
|
IETCCC |
|
1:15.49 |
- |
| 18 |
Irwin, Kailee G (13)
|
IETCCC |
1:16.66 |
1:16.08 |
-0.58 |
| 19 |
Vetter, Isabelle A (13)
|
IETCCC |
1:24.44 |
1:16.51 |
-7.93 |
| 20 |
Fortune, Jaiden C (14)
|
IESAS |
1:20.06 |
1:21.95 |
1.89 |
|
Sawyer, Molly B (13)
|
SRBY |
1:00.30 |
DQ |
- |