| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Nguyen, Leyna M (12)
|
CAYOC |
34.85 |
35.55 |
0.70 |
| 2 |
Kirkwood-Sturzenacke, Kiera (12)
|
CAFFY |
38.70 |
37.52 |
-1.18 |
| 3 |
Molina, Camila (11)
|
CAYOC |
41.67 |
40.18 |
-1.49 |
| 4 |
Lopez, Samantha (11)
|
CAWVYD |
40.46 |
40.96 |
0.50 |
| 4 |
Czarnecka, Katherine H (12)
|
CAFFY |
44.06 |
40.96 |
-3.10 |
| 6 |
Worku, Leah (12)
|
CAWYO |
40.77 |
41.50 |
0.73 |
| 7 |
Ye, Tiffany (12)
|
CABUR |
42.23 |
42.05 |
-0.18 |
| 8 |
Granados, Hailey (12)
|
CAWVYD |
43.69 |
43.22 |
-0.47 |
| 9 |
Der Avanasian, Amy (11)
|
CABUR |
43.91 |
43.41 |
-0.50 |
| 10 |
Brio, Iliana (11)
|
CABUR |
45.64 |
43.78 |
-1.86 |
| 11 |
Bozan, Allena (12)
|
CAGWY |
46.37 |
44.25 |
-2.12 |
| 12 |
Backes, Kimberly (12)
|
CADRGN |
46.24 |
45.92 |
-0.32 |
| 13 |
Ahmed, Seneam (12)
|
CAWYO |
48.62 |
46.40 |
-2.22 |
| 14 |
Su, Emily (12)
|
CAGWY |
45.83 |
46.61 |
0.78 |
| 15 |
Scott, Isabelle R (11)
|
CAWYO |
48.47 |
46.91 |
-1.56 |
| 16 |
Song, Isabel (12)
|
CAFFY |
47.33 |
47.11 |
-0.22 |
| 17 |
Chiou, Chloe (11)
|
CACKFY |
50.11 |
48.26 |
-1.85 |
| 18 |
Williams, Beatrice (12)
|
CACKFY |
|
48.53 |
- |
| 19 |
Dineshbabu Usharani, Mridhul (11)
|
CABUR |
54.91 |
50.57 |
-4.34 |
| 20 |
Klein, Oksana (11)
|
CAWVYD |
|
52.69 |
- |
| 21 |
Wagle, Sharvari (12)
|
CAWVYD |
|
53.69 |
- |
|
Kusano, Erika (12)
|
CAWYO |
47.07 |
DQ |
- |