| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Holmquist, Charlotte S (13)
|
PVRY |
33.77 |
32.98 |
-0.79 |
| 2 |
Arvi, Rachel (13)
|
MDHFY |
36.05 |
35.29 |
-0.76 |
| 3 |
Hutchcroft, Josie R (14)
|
PVPAC |
37.55 |
35.92 |
-1.63 |
| 4 |
Parsons, Kiera K (14)
|
PVPAC |
41.11 |
35.94 |
-5.17 |
| 5 |
Trute, Isabella M (14)
|
MDHFY |
36.21 |
37.22 |
1.01 |
| 6 |
Manela, Joanna R (13)
|
PVPAC |
49.61 |
37.84 |
-11.77 |
| 7 |
Gebhards, Olivia E (13)
|
PVRY |
39.59 |
38.23 |
-1.36 |
| 8 |
Arseculeratne, Maryn A (13)
|
PVRY |
38.08 |
38.63 |
0.55 |
| 9 |
Garcia, Julia S (15)
|
PVPAC |
|
38.89 |
- |
| 10 |
Shanks, Ava J (14)
|
PVPAC |
|
44.45 |
- |
| 11 |
McClintock, Jennifer R (14)
|
MDHFY |
46.28 |
46.64 |
0.36 |
|
Hudson, Ava K (18)
|
MDHFY |
34.45 |
NS |
- |