| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rieger, Noa (14)
|
PVHEAL |
29.94 |
28.87 |
-1.07 |
| 2 |
Williams, Sena T (14)
|
PVFAST |
28.13 |
29.01 |
0.88 |
| 3 |
Brinkley-Tyson, Malaijah A (14)
|
PVDCPR |
30.01 |
29.63 |
-0.38 |
| 4 |
Goodweather, Isabelle L (15)
|
PVDCPR |
29.90 |
30.00 |
0.10 |
| 5 |
Salemi, Sofia A (15)
|
PVDCPR |
31.48 |
30.39 |
-1.09 |
| 6 |
Yang, Julia (15)
|
PVMSSC |
30.35 |
30.54 |
0.19 |
| 7 |
Jones, Sofia K (14)
|
PVMSSC |
30.76 |
30.59 |
-0.17 |
| 8 |
Faye, Jaelyn Y (14)
|
PVDCPR |
29.44 |
31.31 |
1.87 |
| 9 |
Rippon, Tru M (14)
|
PVFAST |
31.90 |
31.34 |
-0.56 |
| 10 |
Ferguson, Teagan E (14)
|
PVDCPR |
32.67 |
32.72 |
0.05 |
| 11 |
Koromah, Amani M (14)
|
PVMSSC |
38.73 |
37.61 |
-1.12 |
| 12 |
Kazim, Fatima (14)
|
PVDCPR |
49.38 |
42.86 |
-6.52 |
|
Powell, Luna (14)
|
PVHEAL |
|
NS |
- |