| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gaston, Aleah S (8)
|
PVNCAP |
39.76 |
38.87 |
-0.89 |
| 2 |
Chong, Finley E (8)
|
PVTIBU |
47.85 |
39.64 |
-8.21 |
| 3 |
LeFaivre, Blair M (8)
|
PVNCAP |
39.57 |
40.79 |
1.22 |
| 4 |
Dolan, Leah (8)
|
PVLIFE |
46.76 |
41.39 |
-5.37 |
| 5 |
Lewis, Jillian A (8)
|
PVTIBU |
|
43.25 |
- |
| 6 |
Hartwell, Poppy A (8)
|
PVTOLL |
|
43.27 |
- |
| 7 |
Hou, Sophia (8)
|
PVJFD |
|
43.91 |
- |
| 8 |
Fioravanti, Claire M (8)
|
PVTOLL |
59.41 |
44.50 |
-14.91 |
| 9 |
Millin, Anna B (7)
|
PVJFD |
|
46.58 |
- |
| 10 |
Tu, Violet (8)
|
PVLIFE |
37.00 |
46.66 |
9.66 |
| 11 |
Achi, Fatimah Al-Zahra K (8)
|
PVPAC |
49.00 |
47.84 |
-1.16 |
| 12 |
Obaidy, Nadia T (8)
|
PVTIBU |
|
48.00 |
- |
| 13 |
Greene, Laila M (8)
|
PVPGPR |
|
48.58 |
- |
| 14 |
Ko, Mikayla A (8)
|
PVLIFE |
37.00 |
49.55 |
12.55 |
| 15 |
Huth, Madeleine M (8)
|
PVTOLL |
|
50.83 |
- |
| 16 |
Guerra, Amiyah A (7)
|
PVPAC |
50.66 |
52.71 |
2.05 |
| 17 |
Smith, Bridget E (7)
|
PVPAC |
1:02.43 |
55.44 |
-6.99 |
| 18 |
Foley, Emily M (7)
|
PVPAC |
|
59.47 |
- |
| 19 |
Ibrahim-Qayyum, Aniya Z (8)
|
PVTIBU |
|
1:00.10 |
- |
| 20 |
Nganga, Sophie M (7)
|
PVPAC |
1:20.69 |
1:13.48 |
-7.21 |