| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Becraft, Isabelle M (10)
|
PVMACH |
42.05 |
43.88 |
1.83 |
| 2 |
Nagle, Audrey R (10)
|
PVMACH |
44.36 |
44.45 |
0.09 |
| 3 |
Maitland, Sydney L (10)
|
PVMACH |
44.73 |
45.60 |
0.87 |
| 4 |
Lim Yang, Nora D (10)
|
PVMACH |
44.58 |
45.68 |
1.10 |
| 5 |
Tohmeh, Tala E (10)
|
PVMACH |
46.03 |
45.92 |
-0.11 |
| 6 |
Kasemsuwannakaeth, Natcha (10)
|
PVMACH |
45.47 |
47.49 |
2.02 |
| 7 |
Lewin, Nya L (10)
|
PVMACH |
48.91 |
48.69 |
-0.22 |
| 8 |
Kudelko, Lia G (10)
|
PVTOLL |
51.63 |
51.08 |
-0.55 |
| 9 |
Li, Samantha W (10)
|
PVMACH |
51.30 |
53.38 |
2.08 |
| 10 |
Wang, Julia M (10)
|
PVMACH |
53.21 |
54.87 |
1.66 |
| 11 |
Cantua, Sofia C (10)
|
PVMACH |
|
1:01.07 |
- |
|
Lissenden, Callahan Y (10)
|
PVMACH |
52.91 |
NS |
- |