| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Aiken, Eleanor R (12)
|
PVAAC |
38.34 |
40.62 |
2.28 |
| 1 |
Shive, Keira X (12)
|
PVNCAP |
44.31 |
42.06 |
-2.25 |
| 1 |
Lopez, Tessa J (11)
|
PVTIBU |
48.56 |
49.00 |
0.44 |
| 2 |
Cauthen, Mary E (11)
|
PVNCAP |
42.66 |
42.88 |
0.22 |
| 2 |
Gropper, Emma H (12)
|
PVAAC |
50.08 |
49.30 |
-0.78 |
| 3 |
Atala, Emma J (12)
|
PVAAC |
43.46 |
43.13 |
-0.33 |
| 3 |
MacDonald, Mia (12)
|
PVWSH |
51.38 |
50.12 |
-1.26 |
| 4 |
Kumar, Indu C (11)
|
PVNCAP |
42.50 |
43.83 |
1.33 |
| 4 |
Meng, Olivia L (11)
|
PVWSH |
|
51.05 |
- |
| 5 |
Mignone-Sankoorikal, Mariell (11)
|
PVNCAP |
44.02 |
45.24 |
1.22 |
| 5 |
Brooks, Casie C (11)
|
PVNCAP |
53.86 |
52.67 |
-1.19 |
| 6 |
Montes de Oca, Freya I (12)
|
PVAAC |
48.21 |
45.61 |
-2.60 |
| 6 |
Tofilon, Sophie J (11)
|
PVNCAP |
50.11 |
52.73 |
2.62 |
| 7 |
Reilly, Clara G (11)
|
PVNCAP |
46.10 |
46.48 |
0.38 |
| 7 |
Anderson, Sophia H (12)
|
PVAAC |
50.87 |
52.78 |
1.91 |
| 8 |
Haeni, Laurel M (12)
|
PVNCAP |
48.27 |
47.20 |
-1.07 |
| 8 |
Wyss, Juliana E (11)
|
PVAAC |
53.46 |
53.05 |
-0.41 |
| 9 |
Deacu, Alexandra J (12)
|
PVLIFE |
47.56 |
48.01 |
0.45 |
| 9 |
Carson, Marleigh M (11)
|
MDACSS |
|
53.39 |
- |
| 10 |
Reeves, Sachi M (12)
|
PVPAC |
48.62 |
48.37 |
-0.25 |
| 10 |
Linhares, Faith B (12)
|
MDACSS |
|
54.23 |
- |
| 11 |
Plett, Evelyn M (12)
|
PVTIBU |
48.55 |
48.96 |
0.41 |
| 11 |
Snow, Jessie K (12)
|
PVAAC |
59.51 |
55.33 |
-4.18 |
| 12 |
Diamond, Wren A (12)
|
PVTIBU |
52.94 |
56.01 |
3.07 |
| 13 |
Waldron, Julia K (12)
|
PVTIBU |
56.89 |
56.51 |
-0.38 |
| 14 |
Keener, Moira V (12)
|
MDACSS |
|
1:04.27 |
- |
| 15 |
Cimino, Arianna (11)
|
PVWSH |
1:02.03 |
1:05.52 |
3.49 |
|
Smith, Emma (11)
|
PVYSS |
1:26.45 |
DQ |
- |
|
Chittiwuttinon, Narin A (11)
|
PVPAC |
|
NS |
- |
|
Vargas, Sophia L (12)
|
PVNCAP |
52.54 |
NS |
- |
|
Paul, Shreya (11)
|
PVLIFE |
1:06.18 |
NS |
- |
|
Hobbs, Samantha S (12)
|
PVAAC |
49.45 |
NS |
- |