| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
McCloskey, Leila F (16)
|
MDROCK |
26.12 |
26.47 |
0.35 |
| 2 |
DeGonia, Olivia G (17)
|
PVTIBU |
26.57 |
26.78 |
0.21 |
| 3 |
Nerantzis, Cecelia A (17)
|
PVTIBU |
26.23 |
27.22 |
0.99 |
| 4 |
Hecht, Emma r (16)
|
NJMB |
27.56 |
28.17 |
0.61 |
| 5 |
Bielec, Jacqueline M (16)
|
PVFAST |
27.90 |
28.20 |
0.30 |
| 6 |
Zimmerman, Gabrielle c (16)
|
NJMB |
26.64 |
28.21 |
1.57 |
| 7 |
Spiotta, Madison A (16)
|
MDROCK |
28.93 |
29.05 |
0.12 |
| 8 |
Maniace, Grace E (16)
|
NJMB |
28.55 |
29.36 |
0.81 |
|
Kovach, Rachel N (17)
|
NJMB |
29.63 |
NS |
- |
|
Stefanyshyn, Isabela M (17)
|
PVNCAP |
29.73 |
NS |
- |