| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Chaddock, Edie M (16)
|
PVRY |
58.21 |
58.22 |
0.01 |
| 2 |
Reeder, Evelyn A (15)
|
MDHFY |
59.59 |
59.28 |
-0.31 |
| 3 |
Anderson, Trinity A (13)
|
MDHFY |
58.87 |
59.62 |
0.75 |
| 4 |
Parhat, Eldana (13)
|
PVRY |
1:01.09 |
59.90 |
-1.19 |
| 5 |
Rios, Juliana J (15)
|
PVPAC |
59.27 |
1:01.20 |
1.93 |
| 6 |
Shah, Amara U (15)
|
PVPAC |
58.91 |
1:01.36 |
2.45 |
| 7 |
Parsons, Kiera K (14)
|
PVPAC |
1:08.39 |
1:03.36 |
-5.03 |
| 8 |
Boardman, Leah J (18)
|
PVPAC |
1:04.32 |
1:03.53 |
-0.79 |
| 9 |
Holmquist, Charlotte S (13)
|
PVRY |
1:03.50 |
1:04.32 |
0.82 |
| 10 |
DaRif, Lilly A (15)
|
PVPAC |
1:01.48 |
1:04.34 |
2.86 |
| 11 |
Carroll, Bailey E (13)
|
PVRY |
1:03.00 |
1:05.11 |
2.11 |
| 12 |
Nelson, Ella S (14)
|
PVPAC |
1:05.29 |
1:05.62 |
0.33 |
| 13 |
DaRif, Addyson R (14)
|
PVPAC |
1:08.99 |
1:08.76 |
-0.23 |
| 14 |
Arseculeratne, Maryn A (13)
|
PVRY |
1:10.59 |
1:10.27 |
-0.32 |
| 15 |
Trute, Isabella M (14)
|
MDHFY |
1:12.60 |
1:14.37 |
1.77 |
| 16 |
Gebhards, Olivia E (13)
|
PVRY |
1:14.09 |
1:16.32 |
2.23 |
| 17 |
Shanks, Ava J (14)
|
PVPAC |
|
1:32.60 |
- |
|
Hudson, Ava K (18)
|
MDHFY |
1:00.29 |
NS |
- |
|
Daly, Elizabeth C (15)
|
PVASA |
1:06.51 |
NS |
- |