| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Puthoff, Claire E (17)
|
OHGMVY |
1:05.28 |
1:04.22 |
-1.06 |
| 2 |
Ericksen, Avery A (16)
|
OHCY |
1:05.26 |
1:04.99 |
-0.27 |
| 4 |
Rhodenbaugh, Hannah R (16)
|
OHCY |
1:05.30 |
1:07.66 |
2.36 |
| 5 |
Carlson, Ashley E (16)
|
OHCFY |
1:07.28 |
1:07.75 |
0.47 |
| 6 |
Wishnok, Miranda R (15)
|
OHCFY |
1:07.60 |
1:07.90 |
0.30 |
| 7 |
Hetman, Lauren E (15)
|
OHCFY |
1:10.08 |
1:08.30 |
-1.78 |
| 8 |
Benbow, Kara E (15)
|
OHCY |
1:06.72 |
1:09.25 |
2.53 |
| 10 |
Eng, Elizabeth C (15)
|
OHCFY |
1:10.36 |
1:10.39 |
0.03 |
| 11 |
Reigle, Kaitlyn A (16)
|
OHGMVY |
1:12.01 |
1:11.18 |
-0.83 |
| 12 |
Phillips, Elizabeth E (15)
|
OHGMVY |
1:16.36 |
1:11.87 |
-4.49 |
| 13 |
Klimo, Molly K (16)
|
OHCY |
1:11.58 |
1:15.63 |
4.05 |
| 14 |
Worthington, Lindsey L (17)
|
OHCY |
1:11.62 |
1:16.59 |
4.97 |
| 15 |
Jones, Olivia R (15)
|
OHCFY |
1:17.05 |
1:16.82 |
-0.23 |
| 17 |
Lentine, Lillian C (15)
|
OHCY |
1:19.96 |
1:19.43 |
-0.53 |
| 18 |
Beck, Brooke A (15)
|
OHGMVY |
1:22.29 |
1:20.06 |
-2.23 |