| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Zubrzycki, Kaitlyn A (14)
|
OHCFY |
2:34.28 |
2:24.02 |
-10.26 |
| 3 |
Heller, Olivia M (14)
|
OHGMVY |
2:43.54 |
2:33.63 |
-9.91 |
| 7 |
Short, Margaret C (13)
|
OHCY |
2:38.57 |
2:35.81 |
-2.76 |
| 8 |
Tenbusch, Julia E (13)
|
OHCY |
2:41.13 |
2:35.97 |
-5.16 |
| 10 |
Gulczinski, Rachel A (13)
|
OHCFY |
2:44.51 |
2:37.43 |
-7.08 |
| 11 |
Eckert, Cassandra M (14)
|
OHCFY |
2:34.75 |
2:38.74 |
3.99 |
| 14 |
Getter, Courtney E (13)
|
OHCFY |
2:49.38 |
2:42.05 |
-7.33 |
| 15 |
Sundermann, Claire E (14)
|
OHCFY |
2:44.33 |
2:42.96 |
-1.37 |
| 16 |
Wang, Sophia B (13)
|
OHCFY |
2:52.30 |
2:44.72 |
-7.58 |
| 18 |
Pugnale, Alyssa L (13)
|
OHCFY |
2:55.65 |
2:52.83 |
-2.82 |