| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Dowell, Lila G (10)
|
NTLAC |
34.98 |
33.71 |
-1.27 |
| 2 |
Wang, Yeyi (8)
|
NTLAC |
35.09 |
34.05 |
-1.04 |
| 3 |
Rabe, Paloma J (10)
|
NTLAC |
51.47 |
34.79 |
-16.68 |
| 4 |
Batra, Pahel A (11)
|
NTLAC |
36.72 |
36.47 |
-0.25 |
| 5 |
Powell, Aubrey S (10)
|
NTLAC |
38.22 |
36.55 |
-1.67 |
| 6 |
Dominguez Davila, Mariana (11)
|
NTLAC |
39.75 |
36.64 |
-3.11 |
| 7 |
Rios, Isabelle A (10)
|
NTLAC |
37.12 |
36.66 |
-0.46 |
| 8 |
Rogers, Drue E (9)
|
NTLAC |
40.62 |
37.77 |
-2.85 |
| 9 |
Crawford, Shiya L (10)
|
NTLAC |
41.49 |
38.39 |
-3.10 |
| 10 |
Hofley, Blake O (10)
|
NTLAC |
39.52 |
39.87 |
0.35 |
| 11 |
Klubben, Chelsea A (8)
|
NTLAC |
42.26 |
40.39 |
-1.87 |
| 12 |
Potter, Jane T (10)
|
NTLAC |
47.48 |
40.53 |
-6.95 |
| 13 |
Van Dusen, Addison L (10)
|
NTLAC |
38.63 |
41.45 |
2.82 |
| 14 |
Hernandez, Kirsten Sophia M (9)
|
NTLAC |
51.76 |
41.50 |
-10.26 |
| 15 |
Medina, Kendall E (11)
|
NTLAC |
44.70 |
41.57 |
-3.13 |
| 16 |
Chen, Kathy L (10)
|
NTLAC |
50.21 |
42.63 |
-7.58 |
| 17 |
Nguyen, Audrey T (8)
|
NTLAC |
48.63 |
44.28 |
-4.35 |
| 18 |
Ye, Olivia L (11)
|
NTLAC |
45.55 |
44.39 |
-1.16 |
| 19 |
Pan-Wang, Alina (9)
|
NTLAC |
|
44.66 |
- |
| 20 |
Johnson, Darcy E (8)
|
NTLAC |
46.36 |
45.81 |
-0.55 |
| 21 |
Xu, Sophie T (8)
|
NTLAC |
47.69 |
47.18 |
-0.51 |
| 22 |
Holladay, Hallie A (7)
|
NTLAC |
|
47.60 |
- |
| 23 |
Gehre, Abigail L (10)
|
NTLAC |
47.04 |
48.22 |
1.18 |
| 24 |
Staudenmyer, Faith M (9)
|
NTLAC |
52.52 |
48.30 |
-4.22 |
| 25 |
Wang, Carrie Z (9)
|
NTLAC |
1:00.30 |
49.07 |
-11.23 |
| 26 |
Swanson, Parker L (9)
|
NTLAC |
49.91 |
49.88 |
-0.03 |
| 27 |
Maddula, Nishika (10)
|
NTLAC |
53.52 |
51.38 |
-2.14 |
| 28 |
Raven, Kirin R (7)
|
NTLAC |
|
56.52 |
- |