| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Shimel, Julia A (12)
|
GUESA |
34.01 |
34.73 |
0.72 |
| 2 |
Rossi, Peyton H (11)
|
GUUN-S |
37.74 |
38.50 |
0.76 |
| 3 |
Albert, Ayla G (12)
|
GUSSS |
38.23 |
38.59 |
0.36 |
| 4 |
Adair, Sydney E (12)
|
GUESA |
39.51 |
38.67 |
-0.84 |
| 5 |
Ekhaese, Iminaghosa (11)
|
GUSSS |
37.35 |
38.99 |
1.64 |
| 6 |
Neal, Brynn A (12)
|
GUPEAK |
41.67 |
39.65 |
-2.02 |
| 7 |
Sherrard, Hailey L (11)
|
GUNOCH |
39.31 |
40.55 |
1.24 |
| 8 |
Nguyen, Elina H (12)
|
GUESA |
42.30 |
41.54 |
-0.76 |
| 9 |
Ledezma, Madison C (12)
|
GUHUNT |
|
41.56 |
- |
| 10 |
Canavan, Megan J (12)
|
GUESA |
42.14 |
41.83 |
-0.31 |
| 11 |
Damon, Carly E (11)
|
GUSSS |
40.34 |
42.13 |
1.79 |
| 12 |
Gapsiewicz, Brianna A (12)
|
GUPEAK |
42.74 |
42.21 |
-0.53 |
| 13 |
Schlorholtz, Ella C (12)
|
GUPFL |
44.89 |
42.97 |
-1.92 |
| 14 |
Lyons, Addison M (12)
|
GUHUNT |
43.76 |
43.99 |
0.23 |
| 15 |
McManus, Taryn M (11)
|
GUPEAK |
46.92 |
44.09 |
-2.83 |
| 16 |
Mayes, Myriam E (12)
|
GUHUNT |
49.69 |
44.39 |
-5.30 |
| 17 |
Burke, Anna E (11)
|
GUPEAK |
44.92 |
44.80 |
-0.12 |
| 18 |
Courtney, Natalie L (12)
|
GUAMBU |
|
46.22 |
- |
| 19 |
Canalito, Eva A (12)
|
GUESA |
49.69 |
46.68 |
-3.01 |
| 20 |
Garcia, Ava E (11)
|
GUNOCH |
46.98 |
46.75 |
-0.23 |
| 21 |
Ren, Jiaqi (11)
|
GUPEAK |
|
47.78 |
- |
| 22 |
Andrade, Mia A (11)
|
GUSSS |
46.15 |
47.96 |
1.81 |
| 23 |
Sood, Anneliese B (11)
|
GUPFL |
51.54 |
48.34 |
-3.20 |
| 24 |
Lucas, Allyson N (12)
|
GUAMBU |
|
51.14 |
- |
| 25 |
Holden, Michelle M (11)
|
GUPFL |
|
52.39 |
- |
| 26 |
Sanchez, Haylen A (11)
|
GUHUNT |
1:03.23 |
52.50 |
-10.73 |
| 27 |
Battles, Amelia J (11)
|
GUAMBU |
55.21 |
52.81 |
-2.40 |
| 28 |
Deacon, Sophia B (12)
|
GUSSS |
1:01.85 |
53.54 |
-8.31 |
| 29 |
Shadow, Hailey R (11)
|
GUAMBU |
1:01.07 |
57.04 |
-4.03 |
|
McKinnon, MacKenzie E (11)
|
GUAMBU |
|
DQ |
- |