| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bonaiti, Naomi S (12)
|
GUAGS |
|
31.60 |
- |
| 2 |
Lopez, Jocelyne (11)
|
GUAMBU |
31.70 |
32.80 |
1.10 |
| 3 |
Wood, Hadley K (12)
|
GUAGS |
34.10 |
33.20 |
-0.90 |
| 4 |
Riddle, Gloria (11)
|
GUAGS |
34.56 |
33.89 |
-0.67 |
| 5 |
Budimlic, Sofia (11)
|
GURICE |
34.74 |
34.86 |
0.12 |
| 6 |
Vose, Haylee E (12)
|
GUAMBU |
33.67 |
36.24 |
2.57 |
| 7 |
Mendez Borges, Isabela H (11)
|
GURICE |
|
37.80 |
- |
| 8 |
Isaac, Jessie (11)
|
GUPLAT |
34.50 |
37.84 |
3.34 |
| 9 |
Hansen, Lauren E (11)
|
GUAMBU |
38.29 |
37.92 |
-0.37 |
| 10 |
Plowman, Alexandra M (11)
|
GUAMBU |
39.32 |
38.71 |
-0.61 |
| 11 |
Nielander, Maegan A (11)
|
GUKATY |
39.92 |
39.41 |
-0.51 |
| 12 |
Molinaro, Liliana E (12)
|
GUPACK |
40.07 |
39.64 |
-0.43 |