| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Chen, Gwyneth E (9)
|
GUEPRC |
|
36.80 |
- |
| 2 |
Groneck, Rylee R (10)
|
GUBTA |
40.26 |
38.41 |
-1.85 |
| 3 |
Bailey, Ava T (10)
|
GUBTA |
39.62 |
38.60 |
-1.02 |
| 4 |
Lopez, Jocelyne (10)
|
GUAMBU |
40.40 |
40.59 |
0.19 |
| 5 |
Tippy, Marin A (9)
|
GUBTA |
39.80 |
42.50 |
2.70 |
| 6 |
Flake, Rilee D (9)
|
GUNEHA |
44.85 |
46.50 |
1.65 |
| 7 |
Quinteros, Emely D (10)
|
GUNEHA |
48.47 |
46.56 |
-1.91 |
| 8 |
Drewery, Laura E (10)
|
GUAMBU |
47.32 |
46.61 |
-0.71 |
| 9 |
Sharp, Cally C (9)
|
GUNEHA |
48.29 |
47.69 |
-0.60 |
| 10 |
Flosser, Natalie H (9)
|
GUSPA |
47.73 |
47.77 |
0.04 |
| 11 |
Do, Julianne Q (9)
|
GUSPA |
50.81 |
47.85 |
-2.96 |
| 12 |
Do, Vivianne V (10)
|
GUSPA |
56.70 |
51.09 |
-5.61 |
| 13 |
Day, Emeline S (10)
|
GUPLAT |
47.04 |
53.54 |
6.50 |
| 14 |
O'Connor, Delaney Q (9)
|
GUPLAT |
54.47 |
54.91 |
0.44 |
| 15 |
Todd, Isabel M (9)
|
GUBTA |
|
59.66 |
- |
| 16 |
Thompson-DeRiso, Karissa A (9)
|
GUBTA |
1:05.07 |
1:01.23 |
-3.84 |
|
Plowman, Alexandra M (10)
|
GUAMBU |
|
DQ |
- |
|
Heineman, Hadley G (10)
|
GUBTA |
57.30 |
NS |
- |