| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rayburn, Myah A (10)
|
GUAQUA |
29.62 |
30.12 |
0.50 |
| 2 |
Welch, Taylor B (10)
|
GUAQUA |
32.64 |
31.26 |
-1.38 |
| 3 |
Schroeder, Madison A (10)
|
GUKATY |
33.54 |
32.54 |
-1.00 |
| 4 |
Deffet, Danielle C (10)
|
GUKATY |
33.28 |
33.21 |
-0.07 |
| 5 |
Deffet, Cathryn E (10)
|
GUKATY |
33.50 |
34.05 |
0.55 |
| 6 |
Freeman, Brooke E (10)
|
GUKATY |
34.80 |
34.16 |
-0.64 |
| 7 |
Hurtado, Sara M (10)
|
GUKATY |
35.26 |
35.49 |
0.23 |
| 8 |
Bock, Evangeline L (10)
|
NTTRAC |
38.80 |
35.90 |
-2.90 |
| 9 |
Rosales/Rodriguez, Alexa (9)
|
GUAMBU |
|
35.98 |
- |
| 10 |
Blackmon, Kyra L (9)
|
GUAMBU |
42.38 |
40.92 |
-1.46 |
| 11 |
Stephenson, Abigail M (9)
|
GUAMBU |
|
46.38 |
- |
| 12 |
Redwing, Merus M (9)
|
NTTRAC |
|
46.64 |
- |
| 13 |
Lavespere, Victoria L (9)
|
GUAMBU |
52.96 |
50.82 |
-2.14 |
| 14 |
Heckendorn, Addy L (9)
|
GUAMBU |
54.05 |
56.88 |
2.83 |