| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rasmussen, Kelsie E (8)
|
GUAGS |
45.46 |
41.60 |
-3.86 |
| 2 |
Glowacki, Madelyn E (8)
|
GUESA |
42.34 |
42.58 |
0.24 |
| 3 |
Aragon Calzada, Luciana (8)
|
GUMAC |
45.58 |
46.52 |
0.94 |
| 4 |
VanVossen, Kathryn G (8)
|
GUMARC |
49.96 |
48.31 |
-1.65 |
| 5 |
Nguyen, Neyla M (8)
|
GUFCST |
59.63 |
49.40 |
-10.23 |
| 6 |
Horak, Haley A (7)
|
GUSSAN |
49.94 |
49.73 |
-0.21 |
| 7 |
Vann, Jillian G (8)
|
GUFCST |
51.63 |
49.82 |
-1.81 |
| 8 |
McDaniel, Kenley E (8)
|
GUMAC |
49.52 |
49.83 |
0.31 |
| 9 |
Simmons, Lucy B (6)
|
GUESA |
52.27 |
51.96 |
-0.31 |
| 10 |
Chen, Mackenzie Y (8)
|
GUFCST |
52.05 |
52.39 |
0.34 |
| 11 |
Treadaway, Caeleigh R (8)
|
GUMAC |
49.72 |
52.89 |
3.17 |
| 12 |
Trivedi, Srishti B (7)
|
GUUFCS |
53.07 |
53.37 |
0.30 |
| 13 |
Knapp, Sofi (7)
|
GUMAC |
1:00.32 |
54.98 |
-5.34 |
| 14 |
Silva, Manuela M (8)
|
GUKATY |
52.72 |
55.18 |
2.46 |
| 15 |
Salazar, Angelina (8)
|
GUPFL |
56.45 |
55.31 |
-1.14 |
| 16 |
Chatman, Kodi V (7)
|
GUFCST |
54.47 |
55.52 |
1.05 |
| 17 |
Blaylock, Madison L (8)
|
GUMARC |
53.35 |
55.54 |
2.19 |
| 18 |
Culberson, Evelyn P (7)
|
GUMAC |
57.95 |
57.16 |
-0.79 |
| 19 |
Coreas, Claire E (7)
|
GUMAC |
53.35 |
58.78 |
5.43 |
| 20 |
Botens, Phoebe H (8)
|
GUKATY |
54.53 |
1:00.21 |
5.68 |
| 21 |
Smyers, Lillie R (7)
|
GUKATY |
1:02.77 |
1:03.23 |
0.46 |
| 22 |
Cogill, Michaela E (7)
|
GUFCST |
1:13.21 |
1:03.86 |
-9.35 |
| 23 |
Han, Victoria D (6)
|
GUFCST |
1:20.03 |
1:05.35 |
-14.68 |
| 24 |
Rosebush, Emilee A (6)
|
GUMAC |
1:08.08 |
1:09.05 |
0.97 |
| 25 |
Tran, BaoGiang N (6)
|
GUFCST |
1:16.29 |
1:10.30 |
-5.99 |
| 26 |
Rao, Thrayee N (8)
|
GUFCST |
1:18.29 |
1:17.97 |
-0.32 |
|
Vann, McKenna J (8)
|
GUFCST |
56.64 |
DQ |
- |
|
Xiang, Maggie (7)
|
GUKATY |
|
NS |
- |
|
Chu, Minyang (8)
|
GUFCST |
1:00.69 |
NS |
- |
|
Vadlamani, Isha (7)
|
GUFCST |
1:29.54 |
NS |
- |