| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Perticari, Quin W (8)
|
MAEAAC |
33.47 |
34.35 |
0.88 |
| 2 |
Nagel, Braxton (8)
|
MARAD |
|
36.82 |
- |
| 3 |
Phelps, Hudson J (8)
|
MAPWAC |
|
37.35 |
- |
| 4 |
Turner, Colten B (8)
|
MASSC |
38.43 |
37.80 |
-0.63 |
| 5 |
Ormsbee, Hunter S (8)
|
MASSC |
|
38.32 |
- |
| 6 |
Linn, Brodie T (8)
|
MARAD |
46.59 |
38.95 |
-7.64 |
| 7 |
Miller, Max B (8)
|
MAUN-M |
|
39.29 |
- |
| 8 |
Gillen, Jude W (8)
|
MASSC |
|
40.59 |
- |
| 9 |
Gregorchuk, Peter T (8)
|
MABSC |
42.33 |
41.91 |
-0.42 |
| 10 |
Davidson, Maurice S (8)
|
MAWA |
|
43.09 |
- |
| 11 |
Roselle, Kallum A (8)
|
MARAD |
|
43.52 |
- |
| 12 |
Andersen, James H (8)
|
MASSC |
45.82 |
44.94 |
-0.88 |
| 13 |
Enakhimion, Makinde N (6)
|
MAEAAC |
46.64 |
45.06 |
-1.58 |
| 14 |
Qi, Ethan N (8)
|
MASSC |
50.86 |
45.22 |
-5.64 |
| 15 |
Xiao, Jason W (8)
|
MAMSA |
|
45.39 |
- |
| 16 |
Ryan, Kevin S (8)
|
MAEAAC |
57.81 |
45.44 |
-12.37 |
| 17 |
Young, Hadden (8)
|
MAGRA |
|
45.59 |
- |
| 18 |
Turner, Peyton C (6)
|
MASSC |
|
45.98 |
- |
| 19 |
Regan, Emmett P (7)
|
MARAD |
|
46.97 |
- |
| 20 |
Daniel, Brian D (8)
|
MASSC |
|
48.14 |
- |
| 21 |
Sullivan, Caiden J (7)
|
MAMSA |
|
50.57 |
- |
| 22 |
Gallagher, Finn R (8)
|
MAGRA |
53.33 |
51.01 |
-2.32 |
| 23 |
Armstrong, Christopher J (7)
|
MAWA |
|
53.78 |
- |
| 24 |
Scime, William E (6)
|
MAGRA |
57.30 |
1:01.27 |
3.97 |
| 25 |
Schalleur, Duke J (7)
|
MAUNGR |
54.61 |
1:01.76 |
7.15 |
| 26 |
Pamidimukkala, Shashi (8)
|
MAACAC |
1:00.00 |
1:06.68 |
6.68 |
| 27 |
Jones, William (7)
|
MAFWAC |
|
1:09.22 |
- |
|
Montgomery, William R (7)
|
MAEAAC |
|
NS |
- |
|
Doerr, Louis V (5)
|
MABWA |
|
NS |
- |
|
Baur, Cole G (8)
|
MABSC |
54.08 |
NS |
- |
|
Chen, Lucas N (8)
|
MAUMAC |
1:10.57 |
NS |
- |