| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wang, Joyce Y (8)
|
PVNCAP |
45.76 |
46.12 |
0.36 |
| 2 |
Nguyen, Nhi P (8)
|
PVNCAP |
48.45 |
47.73 |
-0.72 |
| 3 |
Vasileva, Danica I (8)
|
PVBWST |
50.34 |
50.29 |
-0.05 |
| 4 |
Mello, Molly J (8)
|
PVBWST |
49.65 |
50.36 |
0.71 |
| 5 |
McKenchie, Marilyn (8)
|
PVMACH |
|
50.95 |
- |
| 6 |
Lippman, Elena R (8)
|
PVMACH |
1:01.23 |
51.42 |
-9.81 |
| 7 |
Campbell-Crawford, Zara A (8)
|
PVNCAP |
59.01 |
52.15 |
-6.86 |
| 8 |
Keh, Kellyanne (8)
|
PVNCAP |
|
52.74 |
- |
| 9 |
Palacio, Isabella (8)
|
PVNCAP |
57.19 |
54.69 |
-2.50 |
| 10 |
Brand, Charlotte G (8)
|
PVNCAP |
|
55.72 |
- |
| 11 |
Parikh, Sonia F (8)
|
PVNCAP |
|
56.61 |
- |
| 12 |
North, Kylie J (8)
|
PVNCAP |
59.87 |
56.65 |
-3.22 |
| 13 |
Roth, Ava G (8)
|
PVMACH |
1:00.77 |
56.88 |
-3.89 |
| 14 |
Smith, Gabriella E (8)
|
PVBWST |
|
58.35 |
- |
| 15 |
Radhakrishnan, Maya (8)
|
PVNCAP |
|
58.99 |
- |
| 16 |
Cloninger, Winnie J (8)
|
PVNCAP |
|
59.59 |
- |
| 17 |
Allen, Kailyn M (8)
|
PVMACH |
1:04.32 |
1:00.11 |
-4.21 |
| 18 |
Camet, Yzabella Grace S (8)
|
PVNCAP |
1:01.75 |
1:00.64 |
-1.11 |
| 19 |
Ahuja, Rayna L (8)
|
PVNCAP |
|
1:01.03 |
- |
| 20 |
Dawley, Evelyn C (8)
|
PVBWST |
|
1:01.38 |
- |
| 21 |
Le, Tuyet-Lan (8)
|
PVNCAP |
|
1:07.82 |
- |
| 22 |
Spencer, Evelyn G (8)
|
PVNCAP |
1:21.26 |
1:09.06 |
-12.20 |
| 23 |
Hammerl, Levi A (8)
|
PVMACH |
1:07.58 |
1:09.10 |
1.52 |
| 24 |
Mamillapalli, Ruthvika S (8)
|
PVMACH |
1:04.11 |
1:09.37 |
5.26 |
| 25 |
Sinning, Ella G (8)
|
PVNCAP |
|
1:09.61 |
- |
|
Baker, Mackenzie M (8)
|
PVNCAP |
|
DQ |
- |