| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Williams, Michael A (17)
|
PVNCAP |
48.27 |
49.19 |
0.92 |
| 2 |
Turner, Riley C (15)
|
PVNCAP |
48.09 |
49.45 |
1.36 |
| 3 |
Maclang, Roell G (16)
|
PVNCAP |
49.73 |
52.58 |
2.85 |
| 4 |
Chen, Alexander F (17)
|
PVNCAP |
51.86 |
53.85 |
1.99 |
| 5 |
Cicero, David A (12)
|
PVNCAP |
53.34 |
54.50 |
1.16 |
| 6 |
Bermudez, Lucas (13)
|
PVNCAP |
55.59 |
55.15 |
-0.44 |
| 7 |
Delaney, Benjamin D (16)
|
PVNCAP |
53.46 |
55.32 |
1.86 |
| 8 |
Bell, Charlotte E (14)
|
PVNCAP |
57.09 |
56.82 |
-0.27 |
| 9 |
Cantlon, Michael C (14)
|
PVNCAP |
58.31 |
57.81 |
-0.50 |
| 10 |
Sherlock, Adele N (16)
|
PVNCAP |
56.00 |
58.20 |
2.20 |
| 11 |
Kim, Jayden H (13)
|
PVNCAP |
56.12 |
59.08 |
2.96 |
| 12 |
Guo, Henry Y (13)
|
PVNCAP |
58.43 |
59.77 |
1.34 |
| 13 |
Everitt, Gage M (13)
|
PVNCAP |
1:06.05 |
1:00.08 |
-5.97 |
| 14 |
Nguyen, Fiona N (13)
|
PVNCAP |
1:01.62 |
1:01.84 |
0.22 |
| 15 |
Matis, Landen W (12)
|
PVNCAP |
1:00.97 |
1:02.46 |
1.49 |
| 16 |
Hannesschlager, William N (13)
|
PVNCAP |
1:03.37 |
1:02.89 |
-0.48 |
| 17 |
Testani, Angela N (13)
|
PVNCAP |
1:02.63 |
1:02.93 |
0.30 |
| 18 |
Lawyer, Audrey E (13)
|
PVNCAP |
1:05.01 |
1:05.17 |
0.16 |
| 19 |
Van Buskirk, Amanda L (12)
|
PVNCAP |
1:11.46 |
1:05.28 |
-6.18 |
| 20 |
Snow, Selena G (13)
|
PVNCAP |
1:04.86 |
1:05.36 |
0.50 |
| 21 |
Boillot, Nora C (14)
|
PVNCAP |
1:07.62 |
1:11.02 |
3.40 |
| 22 |
Pham, Ivy V (11)
|
PVNCAP |
1:15.67 |
1:15.91 |
0.24 |
| 23 |
Torrico, Peter K (11)
|
PVNCAP |
1:12.61 |
1:19.10 |
6.49 |