| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Pinedo, Anastasia J (8)
|
PVPM |
22.82 |
21.16 |
-1.66 |
| 2 |
Qin, Zhiyue Z (8)
|
PVNCAP |
22.80 |
21.86 |
-0.94 |
| 3 |
Radford, Gabrielle L (7)
|
PVNCAP |
20.84 |
21.90 |
1.06 |
| 4 |
Evankovich, Avery A (8)
|
PVPM |
27.45 |
22.67 |
-4.78 |
| 5 |
Washburn, Rosalia G (8)
|
PVNCAP |
22.00 |
22.95 |
0.95 |
| 6 |
Sanglier, Chloe A (7)
|
PVPM |
23.67 |
24.12 |
0.45 |
| 7 |
Spencer, Evelyn G (7)
|
PVNCAP |
26.84 |
26.35 |
-0.49 |
| 8 |
Nelson, Zoe J (8)
|
PVPM |
27.13 |
27.11 |
-0.02 |
| 9 |
Cook, Penelope R (7)
|
PVPM |
29.83 |
30.87 |
1.04 |
| 10 |
Duan, Sarah S (6)
|
PVNCAP |
35.74 |
33.32 |
-2.42 |
| 11 |
Forde, Madison B (8)
|
PVPM |
|
35.56 |
- |
| 12 |
Bryan, Audrey G (8)
|
PVNCAP |
50.03 |
37.53 |
-12.50 |
| 13 |
Lannoye, Abigail J (8)
|
VATSU |
33.49 |
42.03 |
8.54 |
| 14 |
DelGrosso, Cara L (8)
|
PVPM |
|
49.00 |
- |
|
McAsey, Caroline J (7)
|
PVPM |
|
DQ |
- |
|
Fatah, Aria S (8)
|
PVPM |
33.68 |
DQ |
- |
|
Cunningham, Kinley C (8)
|
PVPM |
|
DQ |
- |
|
Camet, Yzabella Grace S (7)
|
PVPM |
|
DQ |
- |