| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Nguyen, Brian A (10)
|
NTLAC |
48.34 |
41.62 |
-6.72 |
| 2 |
Futrell, Aleksander J (10)
|
NTLAC |
43.61 |
42.43 |
-1.18 |
| 3 |
Ambat, Jonathan S (10)
|
NTLAC |
44.58 |
43.08 |
-1.50 |
| 4 |
Green, Gavin L (10)
|
NTLAC |
45.19 |
43.40 |
-1.79 |
| 5 |
Gilliland, Beckett W (10)
|
NTLAC |
44.01 |
43.87 |
-0.14 |
| 6 |
Maskey, Arlan (9)
|
NTLAC |
45.28 |
44.57 |
-0.71 |
| 7 |
Thayravanh, Austin A (10)
|
NTLAC |
51.43 |
47.38 |
-4.05 |
| 8 |
Malik, Divay (10)
|
NTLAC |
49.08 |
47.40 |
-1.68 |
| 9 |
Tran, Aiden J (10)
|
NTLAC |
49.10 |
48.26 |
-0.84 |
| 10 |
Hodson, Kaden R (10)
|
NTLAC |
46.32 |
48.34 |
2.02 |
| 11 |
Rojas, Moises A (10)
|
NTMAC |
|
50.68 |
- |
| 12 |
Artiles, Joshua T (10)
|
NTLAC |
1:00.90 |
53.12 |
-7.78 |
| 13 |
Gomez, Evan A (10)
|
NTMAC |
54.80 |
54.20 |
-0.60 |
| 14 |
Srivastava, Aarav N (9)
|
NTLAC |
55.50 |
56.33 |
0.83 |
| 15 |
Coffey, James R (9)
|
NTLAC |
55.24 |
57.14 |
1.90 |
| 16 |
Kirksey, Stephen J (9)
|
NTMAC |
1:00.00 |
57.89 |
-2.11 |
| 17 |
Pelusi, Scott T (9)
|
NTMAC |
58.90 |
59.63 |
0.73 |
| 18 |
Fan, Larry X (10)
|
NTLAC |
53.62 |
1:01.76 |
8.14 |
| 19 |
Adhikari, Aaryav (9)
|
NTLAC |
|
1:04.47 |
- |
| 20 |
Najera, Alejandro M (10)
|
NTMAC |
1:06.38 |
1:05.18 |
-1.20 |
| 21 |
Westbrook, Alexander S (9)
|
NTMAC |
|
1:07.32 |
- |
|
Schneider, Sean E (9)
|
NTLAC |
|
NS |
- |