| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Clift, Elise J (14)
|
NTMAC |
54.60 |
55.48 |
0.88 |
| 2 |
Do, Natalie D (14)
|
NTMAC |
55.42 |
56.87 |
1.45 |
| 3 |
Louden, Ryan M (14)
|
NTMTRO |
58.13 |
1:00.34 |
2.21 |
| 4 |
Le, Valerie T (13)
|
NTMAC |
59.94 |
1:01.49 |
1.55 |
| 5 |
Mills, Ashlyn M (14)
|
NTMAC |
1:01.32 |
1:03.79 |
2.47 |
| 6 |
Satterfield, Corah G (13)
|
NTMTRO |
1:00.48 |
1:03.97 |
3.49 |
| 7 |
Titus, Leighton E (14)
|
NTMAC |
1:03.43 |
1:03.98 |
0.55 |
| 8 |
Constantine, Katherine E (14)
|
NTMARS |
1:04.69 |
1:04.08 |
-0.61 |
| 9 |
Luong, Ava T (13)
|
NTMTRO |
1:04.08 |
1:04.27 |
0.19 |
| 10 |
Daniels, Emily R (13)
|
NTMAC |
1:05.79 |
1:07.93 |
2.14 |
|
Bolte, Alexis L (14)
|
NTLTSD |
1:03.91 |
NS |
- |
|
Nabi, Amelie (13)
|
NTLTSD |
1:04.14 |
NS |
- |
|
Unruh, Leena M (13)
|
NTMAC |
1:09.96 |
NS |
- |
|
Barrington, Sofia I (13)
|
NTMARS |
59.05 |
NS |
- |
|
Gyure, Katherine G (13)
|
NTMTRO |
1:04.90 |
NS |
- |