| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gerard, Reagan E (17)
|
NTLAC |
27.02 |
26.55 |
-0.47 |
| 2 |
Silva, Catarina J (16)
|
NTMAC |
27.71 |
28.70 |
0.99 |
| 3 |
Robaina, Kailee (15)
|
NTMAC |
26.80 |
28.71 |
1.91 |
| 4 |
Cisneros-Siller, Ana S (16)
|
NTMAC |
27.27 |
29.02 |
1.75 |
| 5 |
Bernal, Alicia R (16)
|
NTMAC |
29.17 |
29.20 |
0.03 |
| 6 |
Miceli, Ariana R (16)
|
NTMAC |
29.69 |
29.34 |
-0.35 |
| 7 |
Gomez, Leila H (15)
|
NTMAC |
29.87 |
30.04 |
0.17 |
| 8 |
Donaldson, Cassandra N (17)
|
NTMAC |
28.93 |
30.81 |
1.88 |
| 9 |
Alvarado, Celeste A (16)
|
NTMAC |
29.69 |
31.26 |
1.57 |
| 10 |
Dinh, Danielle S (15)
|
NTLAC |
32.15 |
31.82 |
-0.33 |
|
Rupert, Myah E (15)
|
NTMAC |
|
NS |
- |
|
Triana, Margo K (17)
|
NTMAC |
28.72 |
NS |
- |