| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hill, Kylee (10)
|
IABUR |
1:04.29 |
1:03.17 |
-1.12 |
| 2 |
Speers, Elizabeth (10)
|
IABUR |
1:08.37 |
1:07.53 |
-0.84 |
| 3 |
Ripke, Jada L (10)
|
IAMACR |
1:11.54 |
1:08.01 |
-3.53 |
| 4 |
Roling, Rebecca (10)
|
IADUBQ |
1:13.98 |
1:12.61 |
-1.37 |
| 5 |
Lindeman, Olivia (10)
|
IACRSP |
1:12.40 |
1:13.31 |
0.91 |
| 6 |
Klinkenborg, Madelyn M (10)
|
IABHCP |
1:09.76 |
1:13.36 |
3.60 |
| 7 |
Prenzlow, Marley K (9)
|
IASPNY |
1:15.39 |
1:14.24 |
-1.15 |
| 8 |
Engelkemier, Birdie J (10)
|
IAMUS |
1:17.36 |
1:14.27 |
-3.09 |
| 9 |
Miller, Kimberlee (10)
|
IANEWT |
1:16.24 |
1:15.12 |
-1.12 |
| 10 |
Folden, Claire E (10)
|
IADSMY |
1:18.93 |
1:16.04 |
-2.89 |
| 11 |
Hamner, Rowan E (10)
|
IASCFY |
1:18.96 |
1:16.68 |
-2.28 |
| 12 |
LaPlante, Madelyn E (10)
|
IASCFY |
1:18.73 |
1:17.95 |
-0.78 |
| 13 |
Kline, Kinley J (9)
|
IADSMY |
1:19.41 |
1:18.54 |
-0.87 |
| 14 |
Pfeiferling, Laney (9)
|
IAKEO |
1:18.16 |
1:18.83 |
0.67 |
| 15 |
Beauchamp, Madeline R (10)
|
IADSMY |
1:18.31 |
1:20.15 |
1.84 |