| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Schomaker, Allison H (11)
|
AMPRA |
2:21.67 |
2:18.20 |
-3.47 |
| 2 |
Bowers, Addison R (12)
|
AMPTSC |
2:33.80 |
2:29.17 |
-4.63 |
| 3 |
Merlack, Madalynn L (12)
|
AMRXA |
3:08.87 |
2:40.21 |
-28.66 |
| 4 |
Berg, Camille R (11)
|
AMSFAC |
2:43.02 |
2:41.17 |
-1.85 |
| 5 |
Mitchell, Karsyn K (11)
|
AMBPR |
2:47.83 |
2:45.15 |
-2.68 |
| 6 |
Willison, Ella G (11)
|
AMPTSC |
3:08.20 |
2:52.31 |
-15.89 |
| 7 |
Stoecklein, Caroline M (11)
|
AMSFAC |
2:57.88 |
2:56.41 |
-1.47 |
| 8 |
Munoz, Kimberly A (11)
|
AMSFAC |
2:44.75 |
2:56.75 |
12.00 |
| 9 |
Morelock, Katelyn (12)
|
AMSFAC |
3:09.17 |
3:20.49 |
11.32 |
| 10 |
Fugh-Brooks, Marley A (12)
|
AMSFAC |
3:27.60 |
3:26.76 |
-0.84 |
| 11 |
Dick, Madeline C (11)
|
AMSFAC |
3:31.97 |
3:48.44 |
16.47 |
| 12 |
Chekka, Rashmita S (12)
|
AMSFAC |
3:48.09 |
3:48.84 |
0.75 |
| 13 |
Khan, Maryam M (12)
|
AMSFAC |
4:06.19 |
4:10.80 |
4.61 |