| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Berg, Madeline M (13)
|
AMSFAC |
26.82 |
25.90 |
-0.92 |
| 2 |
Munoz, Kirsten D (13)
|
AMSFAC |
27.46 |
27.42 |
-0.04 |
| 3 |
Pietropaolo, Sophia G (13)
|
AMPRA |
27.76 |
27.51 |
-0.25 |
| 4 |
Schomaker, Anna J (13)
|
AMPRA |
27.41 |
28.08 |
0.67 |
| 5 |
Roig, Poppy A (13)
|
AMUN |
28.03 |
28.18 |
0.15 |
| 6 |
Gregorowicz, Abigail S (13)
|
AMPRA |
29.19 |
29.57 |
0.38 |
| 7 |
Dietiker, Jasmine F (14)
|
AMPTSC |
29.83 |
30.13 |
0.30 |
| 8 |
Uzelac, Jillian L (13)
|
AMRXA |
30.63 |
31.07 |
0.44 |
| 9 |
Sabanovic, Ella (13)
|
AMBPR |
34.12 |
32.65 |
-1.47 |
| 10 |
Conway, Mackenzie M (13)
|
AMBPR |
36.69 |
34.35 |
-2.34 |
| 11 |
Koutavas, Sophia M (14)
|
AMSFAC |
37.00 |
36.29 |
-0.71 |
| 12 |
Brilhart, Lauren K (13)
|
AMUN |
36.06 |
37.37 |
1.31 |
|
Prasanth Reeja, Gourisree (13)
|
AMSFAC |
38.44 |
NS |
- |