| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
McCowin, Hazel (10)
|
AMHAAC |
3:25.40 |
3:08.88 |
-16.52 |
| 2 |
Fisher, Carrie J (10)
|
AMSFAC |
3:22.10 |
3:14.73 |
-7.37 |
| 3 |
Reubi, Natalie R (10)
|
AMANSC |
3:26.76 |
3:15.17 |
-11.59 |
| 4 |
Zakrzewski, Brinley C (10)
|
AMWAAC |
3:26.00 |
3:18.02 |
-7.98 |
| 5 |
Stoecklein, Caroline M (10)
|
AMSFAC |
3:21.98 |
3:24.68 |
2.70 |
| 6 |
Masztak, Evey A (9)
|
AMANSC |
3:25.75 |
3:25.41 |
-0.34 |
| 7 |
Munoz, Kimberly A (10)
|
AMSFAC |
3:31.63 |
3:28.03 |
-3.60 |
| 8 |
Keeley, Zoey A (10)
|
AMWAAC |
3:17.21 |
3:28.12 |
10.91 |
| 9 |
Barnes, Cloey U (9)
|
AMSFAC |
3:58.19 |
3:30.21 |
-27.98 |
| 10 |
Piatek, Emma G (9)
|
AMPTSC |
3:54.75 |
3:30.97 |
-23.78 |
| 11 |
Donnelly, Gabrielle F (10)
|
AMMOON |
4:33.10 |
3:33.28 |
-59.82 |
| 12 |
Cornell, Olivia R (10)
|
AMWAAC |
4:00.34 |
3:34.85 |
-25.49 |
| 13 |
Osmon, Symrin L (10)
|
AMSFAC |
4:06.19 |
3:42.72 |
-23.47 |
| 14 |
Tropeck, Allison B (9)
|
AMWAAC |
4:00.00 |
3:48.17 |
-11.83 |
|
Kozar, Elizabeth G (10)
|
AMSHAQ |
3:43.88 |
DQ |
- |