| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rodriguez, Alejandro D (11)
|
AMPTSC |
35.58 |
36.68 |
1.10 |
| 2 |
Wang, Kevin (11)
|
AMSFAC |
37.80 |
37.52 |
-0.28 |
| 3 |
Goff, Nathaniel W (12)
|
AMSFAC |
40.08 |
38.18 |
-1.90 |
| 4 |
Kubistek, Cade E (11)
|
AMNAC |
37.48 |
38.34 |
0.86 |
| 5 |
Wiggers, Brayden G (12)
|
AMSFAC |
37.17 |
38.38 |
1.21 |
| 6 |
Udumula, Venkata Sohan K (12)
|
AMSFAC |
39.42 |
38.45 |
-0.97 |
| 7 |
Young, Nicklas A (12)
|
AMRACE |
41.57 |
40.52 |
-1.05 |
| 8 |
Thomas, Roman G (11)
|
AMRXA |
41.87 |
41.24 |
-0.63 |
| 9 |
Truong, Vinh Q (11)
|
AMNAC |
43.14 |
41.94 |
-1.20 |
| 10 |
White, Anderson C (12)
|
AMSFAC |
45.80 |
43.13 |
-2.67 |
| 11 |
Lou, Xinyu (11)
|
AMSFAC |
49.19 |
44.24 |
-4.95 |
| 12 |
Gonugunta, Sandilya D (11)
|
AMSFAC |
50.01 |
48.93 |
-1.08 |
| 13 |
Juluru, Chandhan (12)
|
AMSFAC |
51.86 |
51.36 |
-0.50 |
| 13 |
Biagini, Davin J (12)
|
AMPRA |
52.58 |
51.36 |
-1.22 |
| 15 |
Culp, Aaron L (12)
|
AMSFAC |
56.50 |
52.23 |
-4.27 |
| 16 |
Bullard, Gavin E (11)
|
AMRXA |
1:04.60 |
52.98 |
-11.62 |
| 17 |
Gillis, Andrew J (11)
|
AMRXA |
1:01.22 |
53.06 |
-8.16 |
|
Baker, Aiden J (11)
|
AMRACE |
1:12.66 |
DQ |
- |