| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Parisi, Michael A (13)
|
AMUN |
33.99 |
31.78 |
-2.21 |
| 2 |
Cheekati, Vibhav A (13)
|
AMSFAC |
32.87 |
32.73 |
-0.14 |
| 3 |
Panizzi, Josiah D (14)
|
AMUN |
36.97 |
33.77 |
-3.20 |
| 4 |
Frederick, Gavin D (13)
|
AMRACE |
39.65 |
36.25 |
-3.40 |
| 5 |
Rosensteel, Logan W (13)
|
AMUN |
47.77 |
36.92 |
-10.85 |
| 6 |
Piatek, Aleksander J (13)
|
AMPTSC |
36.86 |
37.29 |
0.43 |
| 7 |
Hickman, Troy J (13)
|
AMUN |
42.80 |
37.68 |
-5.12 |
| 8 |
Tarbert, Christopher J (13)
|
AMBPR |
39.37 |
38.23 |
-1.14 |
| 9 |
Motlakunta, Rohit (14)
|
AMSFAC |
45.74 |
41.36 |
-4.38 |
| 10 |
Sundaresh Babu, Riswanth Har (14)
|
AMBPR |
43.13 |
42.49 |
-0.64 |
| 11 |
Pietro, Joseph C (13)
|
AMRACE |
48.20 |
44.39 |
-3.81 |