| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Connelly, Reagan E (12)
|
AMPRA |
2:25.70 |
2:27.87 |
2.17 |
| 2 |
Catlin, Eliza C (12)
|
AMUN-M |
2:43.28 |
2:32.02 |
-11.26 |
| 3 |
Schomaker, Anna J (12)
|
AMPRA |
2:31.95 |
2:35.22 |
3.27 |
| 4 |
Shafer, Riley A (11)
|
AMPRA |
2:38.20 |
2:39.13 |
0.93 |
| 5 |
Schomaker, Allison H (11)
|
AMPRA |
2:48.58 |
2:42.22 |
-6.36 |
| 6 |
Dudkowski, Abigail E (12)
|
AMPRA |
2:47.46 |
2:46.98 |
-0.48 |
| 7 |
Drew, Abigail E (12)
|
AMUN-M |
3:14.21 |
2:47.64 |
-26.57 |
| 8 |
Liu, Stacy R (11)
|
AMPTSC |
2:46.89 |
2:49.25 |
2.36 |
| 9 |
Yurocko, Teagan L (11)
|
AMPRA |
3:04.57 |
2:56.71 |
-7.86 |
| 10 |
Bowers, Addison R (12)
|
AMPTSC |
3:00.49 |
2:57.76 |
-2.73 |
| 11 |
Byars, Adeline F (11)
|
AMNAC |
3:20.83 |
3:05.86 |
-14.97 |
| 12 |
Berg, Camille R (11)
|
AMSFAC |
3:34.57 |
3:13.48 |
-21.09 |
| 13 |
Gigante, Lucca A (12)
|
AMSFAC |
3:22.19 |
3:15.75 |
-6.44 |
| 14 |
Stoecklein, Caroline M (11)
|
AMSFAC |
3:21.98 |
3:15.93 |
-6.05 |
|
Pietropaolo, Sophia G (12)
|
AMPRA |
2:45.67 |
NS |
- |
|
Olson, Catherine (12)
|
AMPTSC |
3:08.47 |
NS |
- |
|
Urling, Alyssa C (11)
|
AMPRA |
3:03.72 |
NS |
- |
|
Zatezalo, Grace S (12)
|
AMPRA |
2:56.67 |
NS |
- |