| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kabala, Ryleigh D (9)
|
AMPRA |
3:23.11 |
2:54.79 |
-28.32 |
| 2 |
Barry, Caroline R (9)
|
AMPRA |
3:23.10 |
2:56.82 |
-26.28 |
| 3 |
Fisher, Carrie J (10)
|
AMSFAC |
2:59.54 |
2:57.17 |
-2.37 |
| 4 |
Stoecklein, Caroline M (10)
|
AMSFAC |
3:08.77 |
2:57.88 |
-10.89 |
| 5 |
Leehr, Abigail S (10)
|
AMPTSC |
3:07.20 |
2:58.08 |
-9.12 |
| 6 |
Conner, Lyla B (10)
|
AMPTSC |
3:05.53 |
3:00.78 |
-4.75 |
| 7 |
Cornell, Olivia R (10)
|
AMWAAC |
3:10.01 |
3:02.36 |
-7.65 |
| 8 |
Garvey, Emma F (10)
|
AMPTSC |
3:21.20 |
3:05.84 |
-15.36 |
| 9 |
Amos, Kelcie M (10)
|
AMPTSC |
3:12.20 |
3:06.65 |
-5.55 |
| 10 |
Adams, Ava R (9)
|
AMBWA |
3:12.43 |
3:11.84 |
-0.59 |
| 11 |
Tropeck, Allison B (9)
|
AMWAAC |
3:21.33 |
3:13.83 |
-7.50 |
| 12 |
Osmon, Symrin L (10)
|
AMSFAC |
3:21.09 |
3:19.87 |
-1.22 |
| 13 |
Barnes, Cloey U (9)
|
AMSFAC |
3:21.19 |
3:21.61 |
0.42 |
| 14 |
Prasad, Anushka T (9)
|
AMSFAC |
3:29.19 |
3:31.90 |
2.71 |
|
Rupert, Rachel L (9)
|
AMPRA |
3:09.11 |
NS |
- |
|
Zakrzewski, Brinley C (10)
|
AMWAAC |
3:42.57 |
NS |
- |