| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Huertas, Andriy A (12)
|
FGMACM |
41.09 |
40.00 |
-1.09 |
| 2 |
Barreto, Joshua (11)
|
UNAT |
40.30 |
41.63 |
1.33 |
| 3 |
Guio, Sebastian (11)
|
FGMWA |
41.70 |
42.04 |
0.34 |
| 4 |
Di Persio, Fabio (12)
|
FGCANE |
48.62 |
42.39 |
-6.23 |
| 5 |
Ruiz, Jesus F (11)
|
FGSDST |
46.37 |
42.78 |
-3.59 |
| 6 |
Silva, Mateo A (12)
|
FGCANE |
52.28 |
44.89 |
-7.39 |
| 7 |
Park, Aiden (12)
|
FGMWA |
45.15 |
45.13 |
-0.02 |
| 8 |
Gonzalez, Roni S (12)
|
FGAJSC |
57.33 |
45.76 |
-11.57 |
| 9 |
Rincon, Carlos J (12)
|
FGMWA |
49.05 |
46.29 |
-2.76 |
| 10 |
Angel, Nathan (11)
|
FGMACM |
45.25 |
46.65 |
1.40 |
| 11 |
Tong, Alexander K (12)
|
FGRANS |
|
47.83 |
- |
| 12 |
Gonzalez, Lucas I (11)
|
FGMACM |
52.39 |
49.09 |
-3.30 |
| 13 |
DePass, Christian M (11)
|
FGAJSC |
|
49.44 |
- |
| 14 |
Forero-Onofre, Daniel (11)
|
FGSDST |
|
50.27 |
- |
| 15 |
Garcia, Leonardo (12)
|
FGOSC |
|
50.43 |
- |
| 16 |
Casarreal, David G (11)
|
FGMACM |
|
50.60 |
- |
| 17 |
Morales, Andres I (11)
|
FGGRSC |
|
51.49 |
- |
| 18 |
Wilson, Nicholas J (11)
|
FGCANE |
|
51.83 |
- |
| 19 |
Agarwal, Ishaan (11)
|
FGGRSC |
56.84 |
51.92 |
-4.92 |
| 20 |
Holt, Oliver E (11)
|
FGMS |
|
52.04 |
- |
| 21 |
Juodzevicius, Joel B (12)
|
UNAT |
56.29 |
53.83 |
-2.46 |
| 22 |
Delvat, Victor P (11)
|
FGCANE |
|
55.16 |
- |
| 23 |
Gonzalez, Keven (11)
|
FGMACM |
53.12 |
55.51 |
2.39 |
| 24 |
Dame, Jarrid W (11)
|
FGCANE |
1:00.86 |
55.95 |
-4.91 |
| 25 |
Villavicencio, Samuel E (12)
|
FGGRSC |
57.55 |
56.93 |
-0.62 |
| 26 |
Tovar, Sebastian R (11)
|
FGGRSC |
54.32 |
59.37 |
5.05 |
| 27 |
Gonzalez, Michael A (11)
|
FGGRSC |
1:00.31 |
1:00.85 |
0.54 |
| 28 |
Agudelo, Camilo (12)
|
FGSFTL |
1:07.92 |
1:01.72 |
-6.20 |
| 29 |
Fonseca, Ian J (12)
|
FGAJSC |
|
1:21.06 |
- |
|
Sun, Solon (11)
|
FGRANS |
|
DQ |
- |
|
Torres, Jose A (11)
|
FGSFTL |
1:00.26 |
NS |
- |
|
Garcia, Alex (11)
|
FGMACM |
|
NS |
- |
|
Tampied, Mateo * (12)
|
FGCANE |
1:09.73 |
NS |
- |