| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Perry, Isabella M (10)
|
FGSOFL |
43.25 |
43.07 |
-0.18 |
| 2 |
La Pietra, Sabina S (8)
|
FGAZFL |
50.63 |
43.51 |
-7.12 |
| 3 |
Strong, Alexandra M (10)
|
FGSOFL |
44.68 |
44.09 |
-0.59 |
| 4 |
La Pietra, Wendy G (6)
|
FGAZFL |
1:02.00 |
44.77 |
-17.23 |
| 5 |
Avila, Sophia A (10)
|
FGSOFL |
44.56 |
45.71 |
1.15 |
| 6 |
Quingatuna, Isabella (10)
|
FGAZFL |
|
46.02 |
- |
| 7 |
Perry, Francesca R (8)
|
FGSOFL |
47.84 |
46.43 |
-1.41 |
| 8 |
Baldwin, Jemma E (8)
|
FGSOFL |
46.15 |
46.65 |
0.50 |
| 9 |
Gomez, Sofia (9)
|
FGSOFL |
48.03 |
47.07 |
-0.96 |
| 10 |
McPherson, Mackenzie A (9)
|
FGSOFL |
46.38 |
47.81 |
1.43 |
| 11 |
Grubbs, Amanda N (10)
|
FGSOFL |
|
47.94 |
- |
| 12 |
Carli, Caterina (10)
|
FGMCDA |
49.88 |
48.39 |
-1.49 |
| 13 |
Rodriguez, Sofia (8)
|
FGAZFL |
55.63 |
48.86 |
-6.77 |
| 14 |
McCranie, Stella (9)
|
FGAZFL |
57.59 |
50.39 |
-7.20 |
| 15 |
Evans, Jordan C (8)
|
FGSOFL |
52.29 |
54.00 |
1.71 |
| 16 |
Wolk, Emily S (9)
|
FGMCDA |
58.28 |
54.60 |
-3.68 |
| 17 |
Henriquez, Jilliam (7)
|
FGAZFL |
|
55.60 |
- |
| 18 |
Slobodchikova, Angelika (10)
|
FLMSAT |
|
56.26 |
- |
| 19 |
Zheng, Elaine L (8)
|
FGAZFL |
|
1:00.10 |
- |
| 20 |
Lydecker, Madison P (10)
|
FGMCDA |
1:06.62 |
1:05.76 |
-0.86 |
|
Martin, Gianna S (8)
|
FGSOFL |
|
NS |
- |