| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gedville, David M (10)
|
WIPX3 |
39.70 |
39.05 |
-0.65 |
| 2 |
Hernandez, Benjamin R (8)
|
WISWAT |
40.74 |
40.00 |
-0.74 |
| 3 |
Susmilch, Joshua E (9)
|
WIPX3 |
39.90 |
40.68 |
0.78 |
| 4 |
Ward-Wade, Colton J (10)
|
WISWAT |
43.07 |
44.36 |
1.29 |
| 5 |
Chetreanu, David A (10)
|
WIPX3 |
43.01 |
44.41 |
1.40 |
| 6 |
Rydelski, Parker J (10)
|
WISWAT |
47.27 |
44.63 |
-2.64 |
| 7 |
Reed, Samuel L (10)
|
WIGYD |
51.96 |
48.14 |
-3.82 |
| 8 |
Burzynski, Braden J (9)
|
WISWAT |
49.15 |
50.80 |
1.65 |
| 9 |
Agger, Dane D (9)
|
WISWAT |
50.68 |
50.96 |
0.28 |
| 10 |
Malinis, Sean Riley J (10)
|
WISWAT |
52.03 |
51.10 |
-0.93 |
| 11 |
Volm, Colin J (10)
|
WILGSC |
|
51.83 |
- |
| 12 |
Salbreiter, Jacob D (9)
|
WISWAT |
51.79 |
52.73 |
0.94 |
| 13 |
Martin, Keegan P (10)
|
WIPX3 |
58.21 |
56.24 |
-1.97 |
|
Mitchell, Reese A (9)
|
WISWAT |
56.64 |
DQ |
- |