| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Linkous, Reagan M (17)
|
WVCM |
5:33.73 |
5:43.96 |
10.23 |
| 2 |
Eiben, Natalie J (14)
|
AMJCCS |
5:41.38 |
5:49.25 |
7.87 |
| 3 |
Urse, Olivia F (15)
|
WVCM |
5:27.55 |
5:49.39 |
21.84 |
| 4 |
Schonbachler, Alyssa E (17)
|
AMJCCS |
5:22.52 |
5:53.60 |
31.08 |
| 5 |
Li, Vivien Y (13)
|
AMANSC |
5:48.25 |
5:53.73 |
5.48 |
| 6 |
McFaden, Amanda J (14)
|
AMJCCS |
5:50.19 |
6:07.71 |
17.52 |
| 7 |
Tieppo, Mikayla R (15)
|
AMANSC |
5:59.79 |
6:12.33 |
12.54 |
| 8 |
Miller, Haley C (16)
|
AMANSC |
6:21.23 |
6:15.06 |
-6.17 |
| 9 |
LaRoche, Francesca L (13)
|
AMWHAT |
6:09.69 |
6:24.95 |
15.26 |
| 10 |
Brown, Mallory * (13)
|
AMMMSA |
6:29.99 |
6:43.95 |
13.96 |
|
Brown, McKenna * (13)
|
AMMMSA |
6:54.99 |
DQ |
- |