| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sens, Natalie R (14)
|
AMRXA |
3:30.99 |
2:41.60 |
-49.39 |
| 2 |
Eiben, Natalie J (16)
|
AMJCCS |
2:36.11 |
2:42.53 |
6.42 |
| 3 |
Owen, Veronika N (14)
|
PVUHAC |
3:00.89 |
2:48.24 |
-12.65 |
| 4 |
Conzemius, Ethena R (17)
|
AMRXA |
3:20.99 |
2:55.41 |
-25.58 |
| 5 |
Cribbs, Mila E (15)
|
AMPRA |
3:17.09 |
3:00.71 |
-16.38 |
| 6 |
Alexander, Gabrielle C (14)
|
AMSVSC |
2:49.20 |
3:02.94 |
13.74 |
| 7 |
Wilson, Alissa L (14)
|
AMSVSC |
2:44.59 |
3:06.94 |
22.35 |
| 8 |
Gauselmann, Madison M (13)
|
AMSRST |
3:14.23 |
3:16.93 |
2.70 |
| 9 |
Massella, Maggie E (16)
|
AMSRST |
3:15.23 |
3:38.04 |
22.81 |
|
Allen, Delaney R (16)
|
AMRXA |
3:00.99 |
DQ |
- |
|
Massella, Mollie J (13)
|
AMSRST |
2:47.05 |
NS |
- |