| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kisow, Olivia P (16)
|
AMANSC |
2:30.95 |
2:38.16 |
7.21 |
| 2 |
Smith, Megan A (16)
|
AMPRA |
2:35.29 |
2:40.90 |
5.61 |
| 3 |
Linkous, Reagan M (17)
|
WVCM |
2:35.17 |
2:42.22 |
7.05 |
| 4 |
Bowser, Lauren (15)
|
AMUN-R |
2:45.30 |
2:42.51 |
-2.79 |
| 5 |
Nemetz, Francesca N (17)
|
AMUNHA |
2:37.69 |
2:43.26 |
5.57 |
| 6 |
Dietz, Isabella M (15)
|
AMANSC |
2:41.05 |
2:43.73 |
2.68 |
| 7 |
Somlo, Stephanie V (15)
|
AMJCCS |
2:36.21 |
2:43.77 |
7.56 |
| 8 |
Urse, Olivia F (15)
|
WVCM |
2:36.39 |
2:44.38 |
7.99 |
| 9 |
Livingston, Olivia M (17)
|
AMJCCS |
2:33.77 |
2:44.95 |
11.18 |
| 10 |
Kauffman, Madison C (15)
|
PVHACC |
2:58.39 |
2:45.15 |
-13.24 |
| 11 |
Tomb, Alyssa J (17)
|
AMPTAC |
2:46.22 |
2:45.18 |
-1.04 |
| 12 |
Philbrick, Olivia L (15)
|
AMUNAT |
2:39.47 |
2:45.49 |
6.02 |
| 13 |
Thomas, Jewel A (15)
|
AMNHA |
2:42.18 |
2:46.05 |
3.87 |
| 14 |
Miller, Haley C (16)
|
AMANSC |
2:53.77 |
2:49.42 |
-4.35 |
| 15 |
Martz, Emma J (15)
|
AMUNHA |
2:42.69 |
2:49.64 |
6.95 |
| 16 |
Rice, Mia L (16)
|
WVCM |
2:45.00 |
2:49.90 |
4.90 |
| 17 |
Glinski, Brooke E (15)
|
AMANSC |
2:58.20 |
2:50.27 |
-7.93 |
| 18 |
Myers, Madalyn G (16)
|
AMJCCS |
2:53.10 |
2:52.34 |
-0.76 |
| 19 |
Mlecko, Brooke L (15)
|
AMJCCS |
3:00.03 |
2:53.29 |
-6.74 |
| 20 |
Wood, Augusta D (15)
|
PVHACC |
3:02.31 |
2:55.52 |
-6.79 |
| 21 |
Hinkson, Michaela L (17)
|
AMANSC |
2:52.47 |
2:57.23 |
4.76 |
| 22 |
Zablocki, Mikayla L (16)
|
AMUN-R |
3:03.37 |
3:01.59 |
-1.78 |
| 23 |
Kooker, Keely D (15)
|
AMPRA |
3:00.11 |
3:03.03 |
2.92 |
| 24 |
Reiter, Josephine G (15)
|
AMJCCS |
3:12.85 |
3:04.75 |
-8.10 |
| 25 |
Forcellini, Cydney R (16)
|
AMGJY |
3:03.14 |
3:17.24 |
14.10 |