| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Berg, Madeline M (12)
|
AMSFAC |
2:30.28 |
2:25.51 |
-4.77 |
| 2 |
Owen, Veronika N (12)
|
PVHACC |
2:35.50 |
2:25.82 |
-9.68 |
| 3 |
Gotaskie, Mary C (12)
|
AMUNJC |
2:32.97 |
2:31.14 |
-1.83 |
| 4 |
Siraj, Hannah (12)
|
PVHACC |
2:46.44 |
2:32.68 |
-13.76 |
| 5 |
Shipe, Bryony (11)
|
AMUN-R |
2:42.41 |
2:36.52 |
-5.89 |
| 6 |
Riehl, Claire E (12)
|
AMUNAT |
2:23.81 |
2:36.74 |
12.93 |
| 7 |
Daltorio, Josephine G (12)
|
AMANSC |
2:51.37 |
2:38.44 |
-12.93 |
| 8 |
Meadows, Tessa M (12)
|
WVCM |
2:51.78 |
2:38.78 |
-13.00 |
| 9 |
Bacu, Claire P (11)
|
AMANSC |
2:45.75 |
2:38.86 |
-6.89 |
| 10 |
Shipe, Dylayn (11)
|
AMUN-R |
2:46.31 |
2:39.28 |
-7.03 |
| 11 |
Hinkson, Danielle N (11)
|
AMANSC |
2:46.73 |
2:39.50 |
-7.23 |
| 12 |
Sterlitz, Amara N (12)
|
AMFAST |
2:44.59 |
2:40.66 |
-3.93 |
| 13 |
De, Lara (11)
|
PVHACC |
3:08.09 |
2:41.69 |
-26.40 |
| 14 |
Stratigos, Melina T (12)
|
AMEFSC |
2:34.25 |
2:44.11 |
9.86 |
| 15 |
Jones, Carissa E (12)
|
AMANSC |
3:11.34 |
2:44.36 |
-26.98 |
| 16 |
Mahon, Shea M (11)
|
AMANSC |
2:48.78 |
2:46.04 |
-2.74 |
| 17 |
Pietropaolo, Sophia G (12)
|
AMPRA |
3:00.27 |
2:48.42 |
-11.85 |
| 18 |
Haglund, Dagny J (12)
|
AMJCCS |
3:29.10 |
2:55.30 |
-33.80 |
| 19 |
Miller, Emily A (11)
|
AMSASC |
3:26.87 |
2:58.08 |
-28.79 |
| 20 |
Donai, Addison D (11)
|
WVCM |
3:04.64 |
2:59.78 |
-4.86 |
| 21 |
Droschak, Madison E (12)
|
AMSHAQ |
3:04.71 |
3:00.79 |
-3.92 |
| 22 |
Melocchi, Grace R (12)
|
AMSASC |
3:16.09 |
3:02.63 |
-13.46 |
| 23 |
Tew, Annabel P (12)
|
AMJCCS |
3:20.19 |
3:07.14 |
-13.05 |
| 24 |
Schaefer, Lillian M (12)
|
AMJCCS |
3:18.46 |
3:08.11 |
-10.35 |
| 25 |
Dubowitz, Talia M (11)
|
AMJCCS |
3:15.19 |
3:15.44 |
0.25 |
| 26 |
Phenizee, Kymiah (12)
|
AMGPAC |
3:58.11 |
3:15.72 |
-42.39 |
| 27 |
Poniatowski, Shelby A (12)
|
AMNHA |
3:50.90 |
3:16.36 |
-34.54 |
| 28 |
Brenner, Camille S (12)
|
AMJCCS |
3:30.19 |
3:19.81 |
-10.38 |
| 29 |
Kunzman, Gabriella S (11)
|
AMJCCS |
3:45.19 |
3:19.85 |
-25.34 |