| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bookwalter, Katelyn E (12)
|
AMSVSC |
2:40.06 |
2:31.07 |
-8.99 |
| 2 |
Puthoff, Madison A (12)
|
AMSTEE |
2:54.48 |
2:46.85 |
-7.63 |
| 3 |
Shipe, Aynsley F (12)
|
AMRGSY |
2:51.92 |
2:49.74 |
-2.18 |
| 4 |
Komoroski, Ava C (12)
|
AMSTEE |
3:10.43 |
2:52.28 |
-18.15 |
| 5 |
McCowin, Hazel L (12)
|
AMSTEE |
3:11.17 |
2:53.43 |
-17.74 |
| 6 |
Motley, Eleanor R (11)
|
AMSTEE |
3:00.93 |
3:03.88 |
2.95 |
| 7 |
Zakrzewski, Brinley C (12)
|
AMWAAC |
3:45.67 |
3:07.93 |
-37.74 |
| 8 |
Keeley, Zoey A (12)
|
AMWAAC |
3:45.67 |
3:20.30 |
-25.37 |
| 9 |
Donnelly, Gabrielle F (12)
|
AMSTEE |
3:59.00 |
3:26.26 |
-32.74 |
| 10 |
Josephson, Mara A (12)
|
AMSVSC |
3:42.45 |
3:27.85 |
-14.60 |
| 11 |
Eichelberger, Isabella K (12)
|
AMNAC |
3:47.54 |
3:40.96 |
-6.58 |
| 12 |
Siemers, Eliza G (11)
|
AMSTEE |
3:45.96 |
4:27.86 |
41.90 |
|
Muralidharan, Risha S (12)
|
AMSTEE |
4:25.10 |
DQ |
- |
|
Morgan, Samantha A (11)
|
AMNAC |
3:39.54 |
NS |
- |
|
Peck, Audrey M (12)
|
AMSTEE |
3:45.00 |
NS |
- |