| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
DiMaio, Mya S (12)
|
AMRGSY |
2:27.29 |
2:24.73 |
-2.56 |
| 2 |
Eroh, Emily A (12)
|
AMRGSY |
2:32.58 |
2:26.91 |
-5.67 |
| 3 |
Bookwalter, Katelyn E (12)
|
AMSVSC |
2:33.09 |
2:28.96 |
-4.13 |
| 4 |
McCowin, Hazel L (12)
|
AMSTEE |
3:08.12 |
2:29.78 |
-38.34 |
| 5 |
Puthoff, Madison A (12)
|
AMSTEE |
2:47.83 |
2:35.00 |
-12.83 |
| 6 |
Muralidharan, Anya S (12)
|
AMSTEE |
2:37.19 |
2:45.42 |
8.23 |
| 7 |
McKiernan, Morgan E (12)
|
AMBPR |
2:51.79 |
2:45.48 |
-6.31 |
| 8 |
Tropeck, Allison B (11)
|
AMWAAC |
3:40.98 |
2:55.14 |
-45.84 |
| 9 |
Piatek, Emma G (11)
|
AMPTSC |
3:03.21 |
3:00.74 |
-2.47 |
|
Danish, Julia N (12)
|
AMPTSC |
3:24.21 |
DQ |
- |
|
Friday, Sophia D (11)
|
AMBPR |
3:00.17 |
NS |
- |
|
Gastgeb, Sofia E (12)
|
AMBPR |
3:17.22 |
NS |
- |
|
Brown, Lindsey R (12)
|
AMSTEE |
2:37.66 |
NS |
- |