| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Noble, Ross M (12)
|
NCYOTA |
31.22 |
31.29 |
0.07 |
| 2 |
Olson, Samuel M (12)
|
NCYOTA |
34.72 |
34.67 |
-0.05 |
| 3 |
Anderson, Case M (12)
|
NCECA |
39.58 |
39.16 |
-0.42 |
| 3 |
Adams, Jackson E (12)
|
NCYOTA |
39.28 |
39.16 |
-0.12 |
| 5 |
Volpe, Andrew S (11)
|
NCYOTA |
42.49 |
40.60 |
-1.89 |
| 6 |
Mori, Axel F (11)
|
NCYOTA |
41.30 |
40.95 |
-0.35 |
| 7 |
Hatfield, Geoffrey T (12)
|
NCECA |
|
41.03 |
- |
| 8 |
Yang, Raymond H (12)
|
NCYOTA |
44.27 |
41.54 |
-2.73 |
| 9 |
Broussard, Kody L (12)
|
NCECA |
|
44.01 |
- |
| 10 |
Wang, Edward (11)
|
NCYOTA |
41.70 |
44.12 |
2.42 |
| 11 |
Johnson, Cameron M (11)
|
NCECA |
48.57 |
44.80 |
-3.77 |
| 12 |
Uveges III, Alfred C (11)
|
NCECA |
|
45.18 |
- |
| 13 |
Hodulik, Stephen F (11)
|
NCYOTA |
48.09 |
46.12 |
-1.97 |
| 14 |
Waters, Austin F (12)
|
NCECA |
48.80 |
46.67 |
-2.13 |
| 15 |
Castillo, Andrew E (11)
|
NCYOTA |
48.25 |
47.65 |
-0.60 |
| 16 |
Jones, Tyler A (11)
|
NCYOTA |
53.07 |
47.82 |
-5.25 |
| 17 |
Lee, Alvin (11)
|
NCYOTA |
49.83 |
49.78 |
-0.05 |
|
Tart, Ethan W (11)
|
NCECA |
44.85 |
NS |
- |