| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ai, Bethany R (12)
|
NCCVAC |
45.76 |
43.37 |
-2.39 |
| 2 |
Johnson, Margaret K (12)
|
NCCVAC |
48.05 |
49.91 |
1.86 |
| 3 |
Rodriquez, Maria V (11)
|
NCMSC |
49.94 |
52.57 |
2.63 |
| 4 |
Lee, Jovie M (11)
|
NCCVAC |
51.08 |
53.03 |
1.95 |
| 5 |
Beltran-Trejo, Nicole (12)
|
NCCVAC |
53.69 |
55.00 |
1.31 |
| 6 |
Van Meter, Claire V (11)
|
NCCVAC |
54.11 |
58.25 |
4.14 |
| 7 |
Lopez-Rodriquez, Naydelin D (12)
|
NCMSC |
1:00.27 |
59.78 |
-0.49 |
| 8 |
Stewart, Leah M (11)
|
NCCVAC |
57.07 |
1:00.44 |
3.37 |
| 9 |
Greene, Jordyn A (11)
|
NCMSC |
59.12 |
1:00.82 |
1.70 |
| 10 |
Watts, Trinity J (11)
|
NCMSC |
1:05.22 |
1:04.44 |
-0.78 |
| 11 |
Ramirez-Soto, Venessa (12)
|
NCRCST |
|
1:19.06 |
- |
|
White, Skyler R (11)
|
NCCVAC |
1:06.11 |
DQ |
- |