| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Berg, Madeline M (12)
|
AMSFAC |
30.43 |
31.01 |
0.58 |
| 2 |
Ricciuti, Giuliana L (12)
|
AMSPY |
31.86 |
32.03 |
0.17 |
| 3 |
Roy, Sylvia M (12)
|
AMMLAC |
26.96 |
32.62 |
5.66 |
| 4 |
Shipe, Bryony (11)
|
AMUN-R |
32.71 |
32.83 |
0.12 |
| 5 |
Munoz, Kirsten D (12)
|
AMSFAC |
33.72 |
33.33 |
-0.39 |
| 6 |
Albu, Helen P (12)
|
AMMLAC |
33.50 |
33.37 |
-0.13 |
| 7 |
Shipe, Dylayn (11)
|
AMUN-R |
33.08 |
33.54 |
0.46 |
| 8 |
Spiess, Emma E (12)
|
AMHD |
32.57 |
33.70 |
1.13 |
| 9 |
Fraundorfer, Alexis R (12)
|
AMBRY |
29.22 |
34.50 |
5.28 |
| 10 |
Newbould, Grace A (12)
|
AMBWA |
35.15 |
34.82 |
-0.33 |
| 11 |
Neemes, Amelia H (12)
|
AMMLAC |
35.50 |
34.92 |
-0.58 |
| 12 |
Petrina, Katherine R (11)
|
AMNGAC |
33.79 |
35.34 |
1.55 |
| 13 |
Moffat, Madison O (12)
|
AMBPR |
35.27 |
36.18 |
0.91 |
| 14 |
Pazuchanics, Kathryn E (12)
|
AMPTSC |
34.90 |
36.21 |
1.31 |
| 15 |
Edner, Callahan E (12)
|
AMBPR |
30.87 |
38.67 |
7.80 |