| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Miller, Henry (14)
|
AMMPAC |
24.40 |
28.34 |
3.94 |
| 2 |
DeMartino, Leonard N (14)
|
AMBPR |
30.78 |
28.58 |
-2.20 |
| 3 |
Cortopassi, Dominic L (14)
|
AMBPR |
27.75 |
28.84 |
1.09 |
| 4 |
Dustevich, Chase T (14)
|
AMMLAC |
28.18 |
28.98 |
0.80 |
| 5 |
Retsch, William M (14)
|
AMHD |
28.01 |
29.00 |
0.99 |
| 6 |
Lydon, Connor J (13)
|
AMNAC |
30.08 |
29.05 |
-1.03 |
| 7 |
Broadhurst, Grant D (13)
|
WVFAST |
30.21 |
29.28 |
-0.93 |
| 8 |
Filer, Aiden S (13)
|
AMPTSC |
28.94 |
29.56 |
0.62 |
| 9 |
Liu, Allan H (14)
|
AMPTSC |
30.31 |
29.63 |
-0.68 |
| 10 |
Graziano, Patton J (13)
|
AMGYST |
29.73 |
30.01 |
0.28 |
| 11 |
Kress, Jackson T (14)
|
AMHD |
29.54 |
30.16 |
0.62 |
| 12 |
Dryden, Bryce A (14)
|
AMBYAC |
29.86 |
30.74 |
0.88 |
| 13 |
Alexander, Conner D (13)
|
AMPTAC |
26.59 |
30.95 |
4.36 |
| 14 |
Blaser, Jack C (14)
|
AMNGAC |
31.00 |
31.99 |
0.99 |
| 15 |
Le, Kenneth B (14)
|
AMMLAC |
31.56 |
32.21 |
0.65 |
| 16 |
Ehmann, Ryan J (14)
|
AMNGAC |
31.79 |
32.96 |
1.17 |
| 17 |
Connell, Christian R (14)
|
AMMLAC |
27.68 |
33.38 |
5.70 |